TAILIEUCHUNG - Lecture Molecular biology (Fifth Edition): Chapter 10 - Robert F. Weaver

In chapter 6 we learned that bacteria have only one RNA polymerase, which makes all three of the familiar RNA types: mRNA, rRNA, and tRNA. In this chapter we will see that three distinct RNA polymerases occur in the nuclei of eukaryotic cells. Each of these is responsible for transcribing a separate set of genes, and each recognizes a different kind of promoter. | Molecular Biology Fifth Edition Chapter 10 Eukaryotic RNA Polymerases and Their Promoters Lecture PowerPoint to accompany Robert F. Weaver Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Multiple Forms of Eukaryotic RNA Polymerase There are at least two RNA polymerases operating in eukaryotic nuclei One transcribes major ribosomal RNA genes One or more to transcribe rest of nuclear genes Ribosomal genes are different from other nuclear genes Different base composition from other nuclear genes Unusually repetitive Found in different compartment, the nucleolus 10- Separation of the 3 Nuclear Polymerases Eukaryotic nuclei contain three RNA polymerases These can be separated by ion-exchange chromatography RNA polymerase I found in nucleolus Location suggests it transcribes rRNA genes RNA polymerases II and III are found in the nucleoplasm 10- Roles of the Three RNA Polymerases Polymerase I makes large rRNA precursor Polymerase II .

• Sinh học
• Molecular biology
• Lecture Molecular biology
• Sinh học phân tử
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• Eukaryotic RNA polymerases
• RNA polymerases
• High Yield cell and molecular biology
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• Molecular biology of cancer
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• Molecular biology techniques
• Identification of human disease genes
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• Molecular biology of the gene
• Chromosome replication
• Meiosis and genetic recombination
• The human nuclear genome
• The human mitochondrial genome
• Molecular cloning methods
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• Directional cloning
• Amino acids
• Molecular tools
• Studying genes
• History of genetics
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• Mendel’s Laws
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• Genetic material
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• Mechanism of transcription
• RNA polymerase structure
• Lac operon
• Bacterial transcription
• Sigma factor switching
• DNA Protein interactions
• The λ family of repressors
• Class II factors
• Transcription activators in eukaryotes
• RNA polymerase II
• Histones in the nucleosome
• Nucleosome structure
• Messenger RNA processing I
• Genes in pieces
• RNA processing II
• Cap structure
• Cytoplasmic polyadenylation
• RNA processing
• Ribosomal RNA processing
• Transfer RNA processing