TAILIEUCHUNG - Lecture Molecular biology: Chapter 21 - Robert F. Weaver

We learned in chapter 20 that DNA replication is at least semidiscontinuous and requires the synthesis of primers before DNA synthesis can begin. We have also learned about some of the major proteins involved in DNA replication in E. coli. Thus, we know that DNA replication is complex and involves more than just a DNA polymerase. This chapter presents a close look at the mechanism of this process in E. coli and in eukaryotes. We will look at the three stages of replication-initiation, elongation, and termination-in a variety of systems. | Molecular Biology Fourth Edition Chapter 21 DNA Replication II: Detailed Mechanism Lecture PowerPoint to accompany Robert F. Weaver Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Initiation Initiation of DNA replication means primer synthesis Different organisms use different mechanisms to make primers Different phages infect E. coli using quite different primer synthesis strategies Coliphages were convenient tools to probe DNA replication as they are so simple they must rely primarily on host proteins to replicate their DNAs 21- Priming in E. coli Primosome refers to collection of proteins needed to make primers for a given replicating DNA Primer synthesis in E. coli requires a primosome composed: DNA helicase DnaB Primase, DnaG Primosome assembly at the origin of replication, oriC uses multi-step sequence 21- Priming at oriC Source: Adapted from DNA Replication, 2/e, (plate 15) by Arthur Kornberg and Tania Baker. 21- .

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• Amino acids
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