TAILIEUCHUNG - A further study on structure and covalent immobilization capacity of poly (tyramine) film

In this report, the poly(Tyramine) (PTyr) film structure was further studied and determined, based on IR (Infrared), XPS (X-Ray Photoelectron Spectroscopy), EQCM (Electrochemical Quartz Crystal Microbalance) results. Especially, the immobilization capacity of PTyr was investigated by EQCM in situ measurement. The results showed the film porosity had increased the immobilization capacity of PTyr, compared to other polymer film with the same NH2 surface concentration. | Journal of Chemistry Vol. 43 2 P. 240 - 244 2005 A FURTHER STUDY ON STRUCTURE AND COVALENT IMMOBILIZATION CAPACITY OF POLY TYRAMINE FILM Received 1st-March-2004 TRAN DAI LAM Faculty of Chemical Technology Hanoi University of Technology SUMMARY In this report the poly Tyramine PTyr film structure was further studied and determined based on IR Infrared XPS X-Ray Photoelectron Spectroscopy EQCM Electrochemical Quartz Crystal Microbalance results. Especially the immobilization capacity of PTyr was investigated by EQCM in situ measurement. The results showed the film porosity had increased the immobilization capacity of PTyr compared to other polymer film with the same NH2 surface concentration. I - INTRODUCTION As we have reported in the previous studies 1 2 the PTyr film presents one primary aliphatic amine per tyramine moiety . a very high surface concentration of reactive sites for immobilization of biomolecules. That is why PTyr films have been widely used in the field of biosensors 3 - 5 . In these previous studies we have also characterized the film surface by different methods such as SEM IR XPS 6 7 . XPS results had provided complementary information on the issue of mechanism. Assuming one or two couplings per monomer we can easily calculate the ratio of carbon number in C-O and C-N linkages to the total carbon number in C-C this ratio would be for one coupling and 1 per two couplings . On the other side the experimental ratio of the intensity peaks of the carbons linked to oxygen C-O and nitrogen C-N on the total intensity peak of aliphatic carbons C-C is . Supposing that ratio C-C could be probably over-estimated due to the surface pollution this value is coherent with the assumption of one coupling per monomer. Table 1 XPS-based electro-oxidation mechanism 1 coupling per monomer 2 couplings per monomer Experimental ratio Carbon types C1s Theoretical ratio Carbon types C1s Theoretical ratio 5C-C 4C-C 1 2C-O 3C-O 1C-N 1C-N Furthermore XPS .

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