TAILIEUCHUNG - Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets

Liquid chromatography mass spectrometry (LC-MS) is a valuable tool in the analysis of proteins and combination of LC-MS with different fragmentation methods provides sequence information on components in complex this work, on-line packed capillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques, . | Eur. J. Biochem. 270 3146-3152 2003 FEBS 2003 doi A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets Margareta Ramstrom1 Charlotte Hagman2 Youri O. Tsybin2 Karin E. Markides1 Per Hakansson2 Albert Salehi3 Ingmar Lundquist3 Rolf Hakanson3 and Jonas Bergquist1 1 Institute of Chemistry Department of Analytical Chemistry 2 Division of Ion Physics The Angstrom Laboratory Uppsala University Sweden 3Department of Pharmacology Institute of Physiological Sciences Lund University Sweden Liquid chromatography mass spectrometry LC-MS is a valuable tool in the analysis of proteins and peptides. The combination of LC-MS with different fragmentation methods provides sequence information on components in complex mixtures. In this work nn-liee accl ed cppillary LC electrospray ionization Fourier transform ion cyclotron resonance MS was combined with two complementary fragmentation techniques . nozzle-skimmer fragmentation and electron capture dissociation for the determination of hormonal peptides in an acid ethanol extract of mouse pancreatic islets. The most abundant pe pt ides those di eri vedl from proinsulin and proglucagon were identified by their masses and additional sequence-tag information established their identities. 1161601 1 the ex peri men ss demon tr fitted the presence of truncated C-peptides des- 25-29 -C-peptide and des- 27-31 -C-peptide. These novel Eddings rlcariy illustrate the potential usefulness of the described technique for on-line sequencing and characterization of peptides in tissue extracts. Keywords liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry electron capture dissociation pancreas peptides. Mass spectrometry is a rapid sensitive and reliable method for the analysis of complex samples. Foriria anmsl orm ion cyclotron resonance mass spectrometry FTICR MS provides the combination of high sensitivity ultra high resolution

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