TAILIEUCHUNG - Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain

The localization of different classes of alcohol dehydro-genases (ADH) in the brain is of great interest because of their role in both ethanol and retinoic acid metabolism. Conflicting data have been reported in the literature. By Northern blot and enzyme activity analyses only class III ADH has been detected in adult brain specimens, while results from riboprobe in situ hybridization indicate class I as well as class IV ADH expression in different regions of the rat brain. | Eur. J. Biochem. 270 1316-1326 2003 FEBS 2003 doi Distribution of class I III and IV alcohol dehydrogenase mRNAs in the adult rat mouse and human brain Dagmar Galter1 Andrea Carmine1 2 Silvia Buervenich1 2 Gregg Duester3 and Lars Olson1 1 Department of Neuroscience and department of Molecular Medicine Clinical Neurogenetics Unit Karolinska Institutet Stockholm Sweden 3OncoDevelopmental Biology Program Burnham Institute La Jolla CA USA The localization of different classes of alcohol dehydrogenases ADH in the brain is of great interest because of their role in both ethanol and retinoic acid metabolism. Conflicting data have been reported in the literature. By Northern blot and enzyme activity analyses only class III ADH has been detected in adult brain specimens while results from riboprobe in situ hybridization indicate class I as well as class IV ADH expression in different regions of the rat brain. Here we have studied the expression patterns of three ADH classes in adult rat mouse and human tissues using radioactive oligonucleotide in situ hybridization. Specificity of probes was tested on liver and stomach control tissue as well as tissue from class IV ADH knock-out mice. Only class III ADH mRNA was found to be expressed in brain tissue of all three investigated species. Particularly high expression levels were found in neurons of the red nucleus in human tissue while cortical neurons pyramidal and granule cells of the hippocampus and dopamine neurons of substantia nigra showed moderate expression levels. Purkinje cells of cerebellum were positive for class III ADH mRNA in all species investigated whereas granular layer neurons were positive only in rodents. The choroid plexus was highly positive for class III ADH while no specific signal for class I or class IV ADH was detected. Our results thus support the notion that the only ADH expressed in adult mouse rat and human brain is class III ADH. Keywords alcohol dehydrogenase in

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