TAILIEUCHUNG - Báo cáo khoa học: Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes

Multi-protein complexes are emerging as important entities of biological activity inside cells that serve to create func-tional diversity by contextual combination of gene products and, at the same time, organize the large number of different proteins into functional units. Many a time, when studying protein complexes rather than individual proteins, the bio-logical insight gained has been fundamental, particularly in cases in which proteins with no previous functional anno-tation couldbe placed intoa functional context derived from their molecular environment . . | Eur. J. Biochem. 270 570-578 2003 FEBS 2003 doi MINIREVIEW Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes Andreas Bauer and Bernhard Kuster Cellzome AG Heidelberg Germany Multi-protein complexes are emerging as important entities of biological activity inside cells that serve to create functional diversity by contextual combination of gene products and at the same time organize the large number of different proteins into functional units. Many a time when studying protein complexes rather than individual proteins the biological insight gained has been fundamental particularly in cases in which proteins with no previous functional annotation could be placed into a functional context derived from their molecular environment . In this minireview we summarize the current state of the art for the retrieval of multi protein complexes by affinity purification and their analysis by mass spectrometry. The advances in technology made over the past few years now enable the study of protein complexes on a proteomic scale and it can be anticipated that the knowledge gathered from such projects will fuel drug target discovery and validation pipelines and that the technology is also going to prove valuable in the emerging field of systems biology. Keyword TAP tandem affinity purification . Protein complexes In the postgenomic era proteins are coming back into focus because it has been realized again that whole genome sequence information alone is not sufficient to explain and predict cellular phenomena as it is largely the proteins that execute and control the majority of cellular activities. While the human genome is estimated to contain approximately 30 000-40 000 genes the corresponding proteome is much more complex. Events such as alternative splicing of genes and post-translational modifications generate a highly diverse set of proteins that could exceed a million distinct molecular .

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