TAILIEUCHUNG - Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression
The vanilloid-like TRP-channel VRL-1 (TRPV2) is a non-selective cation channel expressed by primary sensory neurons and non-neuronal tissues [Caterina, ., Rosen, ., Tominaga, M., Brake, and Julius, D. (1999) Nature398, 436–441]. It is one of the six members of the vanilloid-like TRP-channel family which is now termed the TRPV family [Montell, G., Birnbaumer, L., Flockerzi, V., Bindels, ., Brutford, ., Caterina, ., Clapham, ., Harteneck, C., Heller, S., Julius, D., Kojima, I., Mori, Y., Penner, R., Prawitt, D., Scharenberg, ., Schultz, G., Shimizu, N. and Zhu, . (2002)Mol. Cell 2, 229–231]. . | Eur. J. Biochem. 270 4264-4271 2003 FEBS 2003 doi Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression Ricarda Jahnel1 Olaf Bender1 Lisa M. Minter1. Mathias Dreaer1 Clemens Gillen2 and Ferdinand Hucho1 1 Arbeitsgruppe Neurochemie Institut fur Chemie-Biochemie Freie Universitat Berlin Germany 2Griinenthal GmbH Aachen Germany The vanilloid-like TRP-channel VRL-1 TRPV2 is a non-selective cation channel expressed by primary sensory neurons and non-neuronal tissues Caterina . Rosen . Tominaga M. Brake and Julius D. 1999 Nature 398 436-441 . It is one of the six members of the vanilloid-like TRP-channel family which is now termed the TRPV family Montell G. Birnbaumer L. Flockerzi V. Bindels . Brutford . Caterina . Clapham . Harteneck C. Heller S. Julius D. Kojima I. Mori Y. Penner R. Prawitt D. Scharenberg . Schultz G. Shimizu N. and Zhu . 2002 Mol. Cell 2 229-231 . As it is a temperature-gated channel VRL-1 appears to be functionally related to VR1. In contrast to VR1 VRL-1 is activated at a higher temperature threshold and it does not respond to capsaicin or protons. Here we describe the expression of VRL-1 in the rat dorsal root ganglion-derived cell line F-11 a hybridoma of mouse neuroblastoma N18TG2 and rat dorsal root ganglion cells. We found by RT-PCR that F-11 cells express not only the rat VRL-1 but also its mouse orthologue in a single cell. The F-11 parental cell line N18TG2 also expressed murine VRL-1. Due to its neuronal character the DRG-derived F-11 cell line provides an experimental system for the study of VRL-1 biochemistry. However one has to be aware that both the mouse and the rat protein are expressed simultaneously. Furthermore we cloned VRL-1 from rat brain and analyzed its glycosylation and localization in comparison to the endogenously expressed protein in F-11 cells. In contrast to the
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