TAILIEUCHUNG - Báo cáo khoa học: Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck

We report experiments to investigate the role of the physio-logically relevant protein tyrosine kinase Lck in the ordered phosphorylation of the T-cell receptorfchain. Six synthetic peptides were designed based on the sequences of the immunoreceptor tyrosine-based activation motifs (ITAMs) of thefchain. Preliminary 1 H-NMRstudies of recombinant f chain suggested that it is essentially unstructured and therefore that peptide mimics would serve as useful models for investigating individual ITAM tyrosines | Eur. J. Biochem. 270 2369-2376 2003 FEBS 2003 doi Investigation of the kinetics and order of tyrosine phosphorylation in the T-cell receptor f chain by the protein tyrosine kinase Lck Hazel R. Housden1 Paul J. S. Skipp1 Matthew P. Crump1 Robert J. Broadbridge1 Tom Crabbe2 Martin J. Perry2 and Michael G. Gore1 1 Division of Biochemistry and Molecular Biology School of Biological Sciences University of Southampton UK 2Celltech Group plc Slough UK We report experiments to investigate the role of the physiologically relevant protein tyrosine kinase Lck in the ordered phosphorylation of the T-cell receptor f chain. Six synthetic peptides were designed based on the sequences of the immunoreceptor tyrosine-based activation motifs ITAMs of the f chain. Preliminary1H-NMR studies of recombinant f chain suggested that it is essentially unstructured and therefore that peptide mimics would serve as useful models for investigating individual ITAM tyrosines. Phosphorylation kinetics were determined for each tyrosine by assaying the transfer of 32P by recombinant Lck on to each of the peptides. The rates of phosphorylation were found to depend on the location of the tyrosine leading to the proposal that Lck phosphorylates the six f chain ITAM tyrosines in the order 1N first 3N 3C 2N 1C 2C last as a result of differences in the amino-acid sequence surrounding each tyrosine. This proposal was then tested on cytosolic recombinant T-cell receptor f chain. After in vitro phosphorylation by Lck the partially phosphorylated f chain was digested with trypsin. Separation and identification of the f chain fragments using LC-MS showed as predicted by the peptide phosphorylation studies that tyrosine 1N is indeed the first to be phosphorylated by Lck. We conclude that differences in the amino-acid context of the six f chain ITAM tyrosines affect the efficiency of their phosphorylation by the kinase Lck which probably contributes to the distinct patterns of .

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