TAILIEUCHUNG - Báo cáo khoa học: Biochemical characterization of the native Kv2.1 potassium channel

Functional diversity of potassium channels in both prokaryotic and euk-aryotic cells suggests multiple levels of regulation. Posttranslational regula-tion includes differential subunit assembly of homologous pore-forming subunits. In addition, a variety of modulatory subunits may interact with the pore complex either statically or dynamically. is a delayed recti-fier potassium channel isolated by expression cloning. | ềFEBS Journal Biochemical characterization of the native potassium channel Jean-Ju Chung and Min Li Department of Neuroscience and High Throughput Biology Center Johns Hopkins University Schoolof Medicine Baltimore MD USA Keywords channels oligomerization potassium proteomics purification Correspondence M. Li Department of Neuroscience and High Throughput Biology Center Johns Hopkins University Schoolof Medicine BRB311 733 North Broadway Baltimore MD 21205 USA Fax 1 410 614 1001 Tel 1 410 614 5131 E-mail minli@ Received 8 January 2005 revised 17 May 2005 accepted 2 June 2005 doi Functional diversity of potassium channels in both prokaryotic and eukaryotic cells suggests multiple levels of regulation. Posttranslational regulation includes differential subunit assembly of homologous pore-forming subunits. In addition a variety of modulatory subunits may interact with the pore complex either statically or dynamically. is a delayed rectifier potassium channel isolated by expression cloning. The native polypeptide has not been purified hence composition of the channel complexes was not well understood. Here we report a biochemical characterization of channel complexes from both recombinant cell lines and native rat brain. The channel complexes behave as large macromolecular complexes with an apparent oligomeric size of 650 kDa as judged by gel filtration chromatography. The molecular complexes have distinct biochemical populations detectable by a panel of antibodies. This is indicative of functional heterogeneity. Despite mRNA distribution in a variety of tissues the native polypeptides are more abundantly found in brain and have predominantly subunits but not homologous subunits. The proteins precipitated by and their physiological relevance are of interest for further investigation. Potassium K channel pore-forming a subunits are by far one of the most diverse groups of .

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