TAILIEUCHUNG - Báo cáo khoa học: Protective effects of endomorphins, endogenous opioid peptides in the brain, on human low density lipoprotein oxidation

Neurodegenerative disorders are associated with oxidative stress. Low den-sity lipoprotein (LDL) exists in the brain and is especially sensitive to oxi-dative damage. Oxidative modification of LDL has been implicated in the pathogenesis of neurodegenerative diseases. Therefore, protecting LDL from oxidation may be essential in the brain. | ềFEBS Journal Protective effects of endomorphins endogenous opioid peptides in the brain on human low density lipoprotein oxidation Xin Lin1 Li-Ying Xue1 Rui Wang1 2 Qian-Yu Zhao1 and Qiang Chen1 1 Department of Biochemistry and Molecular Biology Schoolof Life Science Lanzhou University China 2 State Key Laboratory for Oxo Synthesis and Selective Oxidation Lanzhou Institute of ChemicalPhysics Chinese Academy of Sciences China Keywords antioxidant endomorphins free radical lipid peroxidation low-density lipoprotein Correspondence R. Wang State Key Laboratory for Oxo Synthesis and Selective Oxidation Lanzhou Institute of ChemicalPhysics Chinese Academy of Sciences Lanzhou 730000 China Fax 86 931 8912561 Tel 86 931 8912567 E-mail wangrui@ Received 3 December 2005 accepted 23 January 2006 doi Neurodegenerative disorders are associated with oxidative stress. Low density lipoprotein LDL exists in the brain and is especially sensitive to oxidative damage. Oxidative modification of LDL has been implicated in the pathogenesis of neurodegenerative diseases. Therefore protecting LDL from oxidation may be essential in the brain. The antioxidative effects of endomorphin 1 EM1 and endomorphin 2 EM2 endogenous opioid peptides in the brain on LDL oxidation has been investigated in vitro. The peroxidation was initiated by either copper ions or a water-soluble initiator 2 2 -azobis 2-amidinopropane hydrochloride AAPH . Oxidation of the LDL lipid moiety was monitored by measuring conjugated dienes thiobarbituric acid reactive substances and the relative electrophoretic mobility. Low density lipoprotein oxidative modifications were assessed by evaluating apoB carbonylation and fragmentation. Endomorphins markedly and in a concentration-dependent manner inhibited Cu2 and AAPH induced the oxidation of LDL due to the free radical scavenging effects of endomorphins. In all assay systems EM1 was more potent than EM2 and L-glutathione a major .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• Crystal structure
• medical analysis
• biochemical studies
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• bacteria
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• environmental medicine