TAILIEUCHUNG - Báo cáo khoa học: Multi-tasking of nonstructural gene products is required for bean yellow dwarf geminivirus transcriptional regulation

Mastreviridae, of the family geminiviridae, possess a monopartite genome and are transmitted by leafhoppers. Bean yellow dwarf dirus (BeYDV) is a mastrevirus which originated from South Africa and infects dicoyledenous plants, a feature unusual for mastreviridae. Previously, the nonstructural proteins Rep and RepA were examined with respect to their independent roles in BeYDV replication. | sFEBS Journal Multi-tasking of nonstructural gene products is required for bean yellow dwarf geminivirus transcriptional regulation Kathleen L. Hefferon1 Yong-Sun Moon2 and Ying Fan3 1 CornellUniversity CornellResearch Foundation Ithaca NY USA 2 Yeungnam University Department of Horticulture Gyeongsan-si Gyeongsangbuk-do Korea 3 CornellUniversity CornellSchoolof Veterinary Medicine Ithaca NY USA Keywords geminivirus gene expression promoter control transactivation Correspondence K. L. Hefferon University of Toronto Center for Virology 25 Willcocks St. Toronto ON Canada M5J3B2 Fax 1 607 254 1015 Tel 1 607 257 1081 E-mail klh22@ Received 21 June 2006 accepted 7 August 2006 doi Mastreviridae of the family geminiviridae possess a monopartite genome and are transmitted by leafhoppers. Bean yellow dwarf dirus BeYDV is a mastrevirus which originated from South Africa and infects dicoyledenous plants a feature unusual for mastreviridae. Previously the nonstructural proteins Rep and RepA were examined with respect to their independent roles in BeYDV replication. This was achieved by placing both gene products under independent constitutive promoter control and examining their effects on replication-competent constructs. In the current study Rep and RepA are examined further for their roles in regulating BeYDV gene expression using a series of replication-incompetent constructs. While both Rep and RepA are found to behave as equally potent inhibitors of complementary-sense gene expression they differ considerably with respect to their abilities to transactivate virion-sense gene expression. Furthermore RepA is identified as playing more than one role in this transactivation process. A nuclear localization domain is identified in Rep which is absent in RepA and Rep-RepA interactions are examined under in vivo conditions. The study concludes with an investigation into the expression strategies of the BeYDV capsid protein. .

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