TAILIEUCHUNG - Báo cáo khoa học: Antifungal effects and mechanism of action of viscotoxin A3

Viscotoxins are cationic proteins, isolated from different mistletoe species, that belong to the group of thionins, a group of basic cysteine-rich peptides of 5 kDa. They have been shown to be cytotoxic to different types of cell, including animal, bacterial and fungal. The aim of this study was to obtain information on the cell targets and the mechanism of action of vis-cotoxin isoform A3 (VtA3). | iFEBS Journal Antifungal effects and mechanism of action of viscotoxin A3 arrola iiiHir i1 2 loeó Antonio Pnwoda1 a fis I III co l l olina1 I a 11 r a do la f anal2 IVIaiucia JIuVIIUI JUoc OIUUIIIU ruvcua Iviaiia LUloa Ivioiina Lauia vie la vaiiai Jose M. Gonzalez-Ros1 Karola Pfuller3 Uwe Pfuller3 and Jose Villalain1 1 Institute de Biologia Molecular y Celular Universidad MiguelHernandez Alicante Spain 2 Institute de Investigaciones Biologicas Universidad Nacionalde Mar delPlata Argentina 3 Institut fur Phytochemie Private Universitat Witten Herdecke GmbH Witten Germany Keywords antifungal cytotoxicity defence mechanisms mistletoe viscotoxins Correspondence J. Villalain Instituto de Biologia Molecular y Celular Universidad MiguelHernandez E-03202 Elche-Alicante Spain Fax 34 966658 758 Tel 34 966658 759 E-mail jvillalain@ Received 1 September 2005 revised 22 October 2005 accepted 31 October 2005 doi Viscotoxins are cationic proteins isolated from different mistletoe species that belong to the group of thionins a group of basic cysteine-rich peptides of w 5 kDa. They have been shown to be cytotoxic to different types of cell including animal bacterial and fungal. The aim of this study was to obtain information on the cell targets and the mechanism of action of viscotoxin isoform A3 VtA3 . We describe a detailed study of viscotoxin interaction with fungal-derived model membranes its location inside spores of Fusarium solani as well as their induced spore death. We show that VtA3 induces the appearance of ion-channel-like activity the generation of H2O2 and an increase in cytoplasmic free Ca2 . Moreover we show that Ca2 is involved in VtA3-induced spore death and increased H2O2 concentration. The data presented here strongly support the notion that the antifungal activity of VtA3 is due to membrane binding and channel formation leading to destabilization and disruption of the plasma membrane thereby supporting a direct role for .

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