TAILIEUCHUNG - Báo cáo khoa học: Study of synthetic peptides derived from the PKI55

Elucidation of the involvement of protein kinase C subtypes in several dis-eases is an important challenge for the future development of new drug tar-gets. We previously identified the PKI55 protein, which acts as a protein kinase C modulator, establishing a feedback loop of inhibition. | ỊFEBS Journal Study of synthetic peptides derived from the PKI55 protein a protein kinase C modulator in human neutrophils stimulated by the methyl ester derivative of the hydrophobic N-formyl tripeptide for-Met-Leu-Phe-OH Rita Qo l ati r i1 Rofi a Fa 1731 3 n o1 I a ra Fra n POCP hotti 2 AH t i a n rx l l ol lir a3 Romo r iiarrini4 n I La ucivauui uulia laizaiaiiu Laia riaiiucouiicui Wiiiaiiu Iviuiiica nei Iiu JUeiiuII Anna Siniscalchi5 and Susanna Spisani2 1 Dipartimento di Medicina Sperimentale e Diagnostica Sezione Genetica Medica Universita degli Studi di Ferrara Italy 2 Dipartimento di Biochimica e Biologia Molecolare University degli Studi di Ferrara Italy 3 Dipartimento di Studi Farmaceutici University di Roma La Sapienza Italy 4 Dipartimento di Scienze Farmaceutiche University degli Studi di Ferrara Italy 5 Dipartimento di Medicina Clinica e Sperimentale Sezione Farmacologia University degli Studi di Ferrara Italy Keywords chemotaxis human neutrophils lysozyme PKC PKI55 Correspondence R. Selvatici Department of Experimental and Diagnostic Medicine MedicalGenetics Section via Fossato di Mortara 74 44100 Ferrara Italy Fax 39 0532 236157 Tel 39 0532 974474 E-mail svr@ Received 16 October 2007 revised 23 November 2007 accepted 28 November 2007 doi Elucidation of the involvement of protein kinase C subtypes in several diseases is an important challenge for the future development of new drug targets. We previously identified the PKI55 protein which acts as a protein kinase C modulator establishing a feedback loop of inhibition. The PKI55 protein is able to penetrate the cell membrane of activated human T-lymphocytes and to inhibit the activity of a b1 and b2 protein kinase C isoforms. The present study aimed to identify the minimal amino acid sequence of PKI55 that is able to inhibit the enzyme activity of protein kinase C. Peptides derived from both C- and N-terminal sequences were synthesized and initially assayed in .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• medical knowledge
• cytoplasm
• analysis of cytoplasmic
• medical research
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• cell proliferation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine