TAILIEUCHUNG - Báo cáo khoa học: Identification and functional characterization of a novel barnacle cement protein

Barnacle attachment to various foreign materials in water is guided by an extracellular multiprotein complex. A 19 kDa cement protein was purified from the Megabalanus rosacement, and its cDNA was cloned and sequenced. The gene was expressed only in the basal portion of the animal, where the histologically identified cement gland is located. | ễFEBS Journal Identification and functional characterization of a novel barnacle cement protein Youhei Urushida1 Masahiro Nakano1 Satoru Matsuda1 Naoko Inoue2 Satoru Kanai2 Naho Kitamura3 Takashi Nishino3 and Kei Kamino1 1 Marine Biotechnology Institute Iwate Japan 2 Pharma Design Inc. Tokyo Japan 3 Department of ChemicalScience Engineering Faculty of Engineering Kobe University Japan Keywords biologicaladhesive extracellular protein holdfast protein protein adsorption sessile organism Correspondence K. Kamino Marine Biotechnology Institute 3-75-1 Heita Kamaishi Iwate 026-0001 Japan Fax 81 193 26 6592 Tel 81 193 26 6584 E-mail Database The nucleotide sequence data are available in the DNA Data Bank of Japan under the accession numbers AB242294 AB242295 and AB242296 Received 20 March 2007 revised 26 June 2007 accepted 29 June 2007 doi Barnacle attachment to various foreign materials in water is guided by an extracellular multiprotein complex. A 19 kDa cement protein was purified from the Megabalanus rosa cement and its cDNA was cloned and sequenced. The gene was expressed only in the basal portion of the animal where the histologically identified cement gland is located. The sequence of the protein showed no homology to other known proteins in the databases indicating that it is a novel protein. Agreement between the molecular mass determined by MS and the molecular weight estimated from the cDNA indicated that the protein bears no post-translational modifications. The bacterial recombinant was prepared in soluble form under physiologic conditions and was demonstrated to have underwater irreversible adsorption activity to a variety of surface materials including positively charged negatively charged and hydrophobic ones. Thus the function of the protein was suggested to be coupling to foreign material surfaces during underwater attachment. Homologous genes were isolated from Balanus albicostatus and B. improvisus .

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