TAILIEUCHUNG - γδ T cells response to Mycobacterium tuberculosis in pulmonary tuberculosis patients using preponderant complementary determinant region 3 sequence

Patients and study design: The study was performed on randomly selected 50 pulmonary tuberculosis patients (mean age, ± , 28 men and 22 women) who had been admitted to the Beijing Tuberculosis and Thoracic Tumor Research Institute during 12 months (April 2008 - April 2009). Pulmonary tuberculosis was diagnosed by the clinical parameters: presence of cough/expectoration, chest X-ray showing infiltrated and /or cavities, a minimum of one positive sputum smear and culture result for acid-fast bacilli. The exclusion criteria were human immunodeficiency virus positivity, diabetes mellitus, pregnancy and immunological or autoimmune diseases (Table I). Ten healthy volunteers (mean age,. | Indian J Med Res 134 September 2011 pp 356-361 Ỵỗ T cells response to Mycobacterium tuberculosis in pulmonary tuberculosis patients using preponderant complementary determinant region 3 sequence Xueyan Xi Xiqin Han Liang Li Zhendong Zhao State Key Laboratory for Molecular Virology and Genetic Engineering Institute of Pathogen Biology Chinese Academy of Medical Sciences Peking Union Medical College Beijing Tuberculosis Thoracic Tumor Research Institute Beijing PR China Received May 18 2010 Background objectives The unique immunological functions of Yổ T lymphocytes to contribute immunity against Mycobacterium tuberculosis attracted interest of researchers. However little is known about the specificity of Ỵổ T cell in tuberculosis patients and the lack of exact tuberculosis antigen recognized by Ỵổ T cells limited its application. The analysis of complementary determinant region CDR 3 sequence characteristic in Ỵổ T cells of tuberculosis patients would contribute to understand the distribution specificity of Ỵổ T cell. In present study we investigated the diversity of the T9 Ổ2 T cell immunorepertoire and analysed the specificity of the expressed CDR3 in pulmonary tuberculosis patients. Methods The total RNA in peripheral blood mononuclear cell of 50 pulmonary tuberculosis patients and 10 healthy controls was extracted. The polymerase chain reaction was used to specifically amplify the CDR3 region of y9 and Ổ2 chain. The PCR products were ligated into the pGEM-T easy vector. The plasmid DNA was sequenced using the ABI3700 and the T7 primer. Results Our findings showed that predominant CDR3 sequence of Ổ2 chain in pulmonary tuberculosis patients was CACDTLVSTDKLIFGKG. The sequence specifically exists in almost all pulmonary tuberculosis patients. The conserved hydrophobic acid residue in 97 positions is present in the YÔ T cell reactive to M. tuberculosis. The length of Ổ2 CDR3 in pulmonary tuberculosis patients has no relation with the disease progress. .

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