TAILIEUCHUNG - Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics

Detection of cancer at early stages that can be treated through surgery is a difficult task. One methodology for cancer biomarker discovery exploits the fact that glycoproteins produced by cancer cells have altered glycan structures, although the proteins themselves are common, ubiquitous, abundant, and familiar. | ỊFEBS Journal REVIEW ARTICLE A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics Hisashi Narimatsu Hiromichi Sawaki Atsushi Kuno Hiroyuki Kaji Hiromi Ito and Yuzuru Ikehara Research Center for MedicalGlycoscience RCMG National institute of Advanced Industrial science and Technology AIST Tsukuba Japan Keywords biomarker glycan MS glyco-biomarker glycogene glycomics glycoproteomics IGOT JCGGDB lectin microarray qPCR array Correspondence H. Narimatsu Research Center for Medical Glycoscience National institute of Advanced Industrial science and Technology AIST 1-1-1 Umezono Tsukuba Ibaraki 305-8568 Japan Fax 81 298 861 3191 Tel 81 298 861 3200 E-mail Received 24 June 2009 Revised 7 September 2009 accepted 9 October 2009 doi Detection of cancer at early stages that can be treated through surgery is a difficult task. One methodology for cancer biomarker discovery exploits the fact that glycoproteins produced by cancer cells have altered glycan structures although the proteins themselves are common ubiquitous abundant and familiar. However as cancer tissue at the early stage probably constitutes less than 1 of the normal tissue in the relevant organ only 1 of the relevant glycoproteins in the serum should have altered gly-can structures. Here we describe our strategy to approach the detection of these low-level glycoproteins a a quantitative real-time PCR array for glycogenes to predict the glycan structures of secreted glycoproteins b analysis by lectin microarray to select lectins that distinguish cancer-related glycan structures on secreted glycoproteins and c an isotope-coded glycosylation site-specific tagging high-throughput method to identify carrier proteins with the specific lectin epitope. Using this strategy we have identified many glycoproteins containing glycan structures that are altered in cancer cells. These candidate glycoproteins were .

• Báo cáo khoa học
• Scientific reports
• scientific research
• biochemistry
• glycoproteomics
• medical research
• immune system
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes