TAILIEUCHUNG - Báo cáo lâm nghiệp: "Micropropagation of hybrid walnut trees (Juglans nigra x Juglans regia) through culture and multiplication of embryos"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Micropropagation of hybrid walnut trees (Juglans nigra x Juglans regia) through culture and multiplication of embryos. | 113s Ann. Sci. For. 1989 46 suppl. 113s-116s Forest Tree Physiology E. Dreyer et al. eds. Elsevier INRA Micropropagation of hybrid walnut trees Juglans nigra X Juglans regia through culture and multiplication of embryos D. Cornu and c. Jay-Allemand Amelioration des Arbres Forestiers INRA Ardon 45160 Olivet France Introduction Walnuts are very valuable trees for nuts and wood production but we see a reduction in the number of logs available for veneer. Although Persian walnut Juglans regia and black walnut J. nigra can be used hybrids between these two species have better growth and wider adaptability. Breeding programs are in progress at INRA Institut National de la Recherche Agronomique Bordeaux and Orleans and they need efficient methods of vegetative propagation. Micropropagation was established in 1984 for Paradox Driver and Kuniyuki 1984 and recently for Persian walnut McGranahan et al. 1988 . Previous works have shown strong effects of ageing and rejuvenation Jay-Allemand et al. 1988 latent contamination low reactivity of buds or meristems on the establishment of mature selected clones. On the contrary with very juvenile material such as embryos it is possible to avoid these problems Jay-Allemand and Cornu 1986 Heile-Sudholt etal. 1986 . The purpose of this study was 1 to estimate the ability of hybrid progeny to be propagated by micropropagation and 2 to improve culture factors acting on shoot development. Materials and Methods This study used half-sib hybrid nuts J. nigra no. 23 3 J re9 a supplied by E Germain INRA Bordeaux collected in September 1987. Embryonic axes 48 were isolated under sterile conditions and then introduced in vitro into the medium defined by McGranahan et al. 1987 . Three main steps have been determined 1 elongation of epicotyls and buds during 3 wk of darkness followed by 2 wk of 16 h of light at 28 C 2 multiplication by transferring nodes from elongated shoots or clusters of buds every 2 wk 16 h light 28 C 3 rooting not presented in

• Báo cáo khoa học
• Báo cáo lâm nghiệp hay
• cách trình bày báo cáo
• báo cáo lâm nghiệp
• công trình nghiên cứu lâm nghiệp
• tài liệu về lâm nghiệp