TAILIEUCHUNG - Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization

We investigated the influence of an epitope from the third intracellular loop (ic3) of the dopamine D2 receptor, which contains adjacent arginine residues (217RRRRKR222), and an acidic epitope from the C-terminus of the dopamine D1 receptor (404EE405) on the receptors’ localization and their interaction. | Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization Sylwia -Lukasiewicz1 Agata Faron-Gorecka2 Jerzy Dobrucki3 Agnieszka Polit1 and Marta Dziedzicka-Wasylewska1 2 1 Department of PhysicalBiochemistry Jagiellonian University Krakow Poland 2 Laboratory of BiochemicalPharmacology Polish Academy of Sciences Krakow Poland 3 Division of CellBiophysics Jagiellonian University Krakow Poland Keywords Arg-rich motif dopamine D1 receptor dopamine D2 receptor FRET GPCR oligomerization Correspondence M. Dziedzicka-Wasylewska Faculty of Biochemistry Biophysics and Biotechnology Jagiellonian University 7 Gronostajowa Street Krakow Poland Fax 48 0I2 664 6902 or 48 012 637 4500 Tel 48 012 664 6122 or 48 012 662 3372 E-mail wasyl@ Received 1 August 2008 revised 19 November 2008 accepted 27 November 2008 doi We investigated the influence of an epitope from the third intracellular loop ic3 of the dopamine D2 receptor which contains adjacent arginine residues 217RRRRKR222 and an acidic epitope from the C-terminus of the dopamine D1 receptor 404EE405 on the receptors localization and their interaction. We studied receptor dimer formation using fluorescence resonance energy transfer. Receptor proteins were tagged with fluorescence proteins and expressed in HEK293 cells. The degree of D D2 receptor heterodimerization strongly depended on the number of Arg residues replaced by Ala in the ic3 of D2R which may suggest that the indicated region of ic3 in D2R might be involved in interactions between two dopamine receptors. In addition the subcellular localization of these receptors in cells expressing both receptors D1-cyan fluorescent protein D2-yellow fluorescent protein and various mutants was examined by confocal microscopy. Genetic manipulations of the Arg-rich epitope induced alterations in the .

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