TAILIEUCHUNG - Báo cáo khoa học: Inhibition of PI3K/Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells

Cellular prion protein (PrP C ), a glycosyl-phosphatidylinositol-anchored membrane protein with unclear physiological function, was previous found to be upregulated in adriamycin (ADR)-resistant gastric carcinoma cell line SGC7901/ADR compared to its parental cell line SGC7901. | Inhibition of PI3K Akt partially leads to the inhibition of PrPC-induced drug resistance in gastric cancer cells Jie Liang Fulin Ge Changcun Guo Guanhong Luo Xin Wang Guohong Han Dexin Zhang Jianhong Wang Kai Li Yanglin Pan Liping Yao Zhanxin Yin Xuegang Guo Kaichun Wu Jie Ding and Daiming Fan State Key Laboratory of Cancer Biology and Xijing Hospitalof Digestive Diseases Fourth Military MedicalUniversity Xi an Shaanxi China Keywords drug resistance gastric cancer P-gp PI3K Akt prion protein Correspondence J. Ding State Key Laboratory of Cancer Biology and Xijing Hospitalof Digestive Diseases Fourth Military MedicalUniversity Xi an 710032 China Fax 86 29 82539041 Tel 86 29 84771504 E-mail dingjie@ D. Fan State Key Laboratory of Cancer Biology and Xijing Hospitalof Digestive Diseases Fourth Military MedicalUniversity Xi an 710032 China Fax 86 29 82539041 Tel 86 29 84775221 E-mail fandaim@ These authors contributed equally to this work Received 1 October 2008 revised 16 November 2008 accepted 24 November 2008 doi Cellular prion protein PrPC a glycosyl-phosphatidylinositol-anchored membrane protein with unclear physiological function was previous found to be upregulated in adriamycin ADR -resistant gastric carcinoma cell line SGC7901 ADR compared to its parental cell line SGC7901. Overexpression of PrPC in gastric cancer has certain effects on drug accumulation through upregulation of P-glycoprotein P-gp which is suggested to play an important role in determining the sensitivity of tumor cells to chemotherapy and is linked to activation of the phosphatidylinositol-3-kinase Akt PI3K Akt pathway. In the present study we further investigate the role of the PI3K Akt pathway in PrPC-induced multidrug-resistance MDR in gastric cancer. Immunohistochemistry and confocal microscope detection suggest a positive correlation between PrPC and phosphorylated Akt p-Akt expression in gastric cancer. Using established stable PrPC

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