TAILIEUCHUNG - Báo cáo khoa học: "Histological investigation of the multiplication step in secondary somatic embryogenesis of Quercus robur L"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Histological investigation of the multiplication step in secondary somatic embryogenesis of Quercus robur L. | 681 Ann. For. Sci. 58 2001 681-690 INRA EDP Sciences 2001 Original article Histological investigation of the multiplication step in secondary somatic embryogenesis of Quercus robur L. Redouane Zegzouti Marie-France Arnould and Jean-Michel Favre Unité Mixte de Recherche INRA-UHP Nancy Interactions arbres micro-organismes Faculté des Sciences BP 239 54506 Vandreuvre-lès-Nancy Cedex France Received 15 February 2000 accepted 20 March 2001 Abstract - Standardized explants composed of hypocotyl and root-tip were prepared from embryonic structures obtained from one em-bryogenic line of Quercus robur L. maintained by regular transfer onto a solidified reference medium composed of the MS mineral solutions glucose M casamino acid and NAA pM . The regeneration capacity from these explants were tested on the reference medium and on 2 alternative media in which the NAA pM was omitted or substituted for a combination of IBA pM BAP pM . Within 30 days 4 types of responses were observed including direct and indirect secondary embryogenesis. In the direct pathway somatic embryos arose from 3-4 epidermal cells following two different modes depending on whether or not the formation of a meristematic mass preceded the initiation of the embryogenic process. In the indirect pathway the embryos were formed from clumps of mitotically active cells included in callus developed within the cortical tissues. Depending on their histological origin the embryos exhibited differences in their structural organization which could influence their potential for further maturation and conversion into viable plantlets. Explants prepared from small translucent embryonic structures were more embryogenic and expressed the direct pathway of secondary embryogenesis at higher frequency than explants prepared from more advanced embryonic structures. On the culture medium without growth regulator direct secondary embryogenesis was the exclusive response whereas on the culture .

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