TAILIEUCHUNG - Enterovirus type 71 2A protease functions as a transcriptional activator in yeast

Abstract Enterovirus type 71 (EV71) 2A protease exhibited strong transcriptional activity in yeast cells. The transcriptional activity of 2A protease was independent of its protease activity. EV71 2A protease retained its transcriptional activity after truncation of 40 amino acids at the N-terminus but lost this activity after truncation of 60 amino acids at the N-terminus or deletion of 20 amino acids at the C-terminus. Thus, the acidic domain at the C-terminus of this protein is essential for its transcriptional activity. Indeed, deletion of amino acids from 146 to 149 (EAME) in this acidic domain lost the transcriptional activity of EV71. | Yang et al. Journal of Biomedical Science 2010 17 65 http content 17 1 65 JOURNAL OF BIOMEDICAL SCIENCE The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan RESEARCH Open Access Enterovirus type 71 2A protease functions as a transcriptional activator in yeast 1 2 1 3 3 13 4 Chee-Hing Yang Hui-Chun Li Jeng-Geng Jiang Che-Fang Hsu Yi-Jen Wang Meng-Jiun Lai 1 Yue-Li Juang Shih-Yen Lo1 3 5 Abstract Enterovirus type 71 EV71 2A protease exhibited strong transcriptional activity in yeast cells. The transcriptional activity of 2A protease was independent of its protease activity. EV71 2A protease retained its transcriptional activity after truncation of 40 amino acids at the N-terminus but lost this activity after truncation of 60 amino acids at the N-terminus or deletion of 20 amino acids at the C-terminus. Thus the acidic domain at the C-terminus of this protein is essential for its transcriptional activity. Indeed deletion of amino acids from 146 to 149 EAME in this acidic domain lost the transcriptional activity of EV71 2A protein though still retained its protease activity. EV71 2A protease was detected both in the cytoplasm and nucleus using confocal microscopy analysis. Coxsackie virus B3 2A protease also exhibited transcriptional activity in yeast cells. As expected an acidic domain in the C-terminus of Coxsackie virus B3 2A protease was also identified. Truncation of this acidic domain resulted in the loss of transcriptional activity. Interestingly this acidic region of poliovirus 2A protease is critical for viral RNA replication. The transcriptional activity of the EV71 or Coxsackie virus B3 2A protease should play a role in viral replication and or pathogenesis. Background Enterovirus type 71 EV71 is the causative agent of several human diseases including hand-foot-and-mouth disease encephalitis and meningitis. EV71 is a singlestranded positive-sense RNA virus which belongs to the .

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