TAILIEUCHUNG - Differential behaviour of normal, transformed and Fanconi’s anemia lymphoblastoid cells to modeled microgravity

Cuccarolo et al. Journal of Biomedical Science 2010, 17:63 Whether microgravity might influence tumour growth and carcinogenesis is still an open issue. It is not clear also if and how normal and transformed cells are differently solicited by microgravity. The present study was designed to verify this issue. Methods: Two normal, LB and HSC93, and two transformed, Jurkat and 1310, lymphoblast cell lines were used as representative for the two conditions. Two lymphoblast lines from Fanconi’s anemia patients group A and C (FA-A and FA-C, respectively), along with their isogenic corrected counterparts (FA-A-cor and FA-C-cor) were also used. Cell. | Cuccarolo et al. Journal of Biomedical Science 2010 17 63 http content 17 1 63 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Differential behaviour of normal transformed and Fanconi s anemia lymphoblastoid cells to modeled microgravity 14t 1 1 Paola Cuccarolo Francesca Barbieri Monica Sancandi Silvia Viaggi Paolo Degan Abstract Background Whether microgravity might influence tumour growth and carcinogenesis is still an open issue. It is not clear also if and how normal and transformed cells are differently solicited by microgravity. The present study was designed to verify this issue. Methods Two normal LB and HSC93 and two transformed Jurkat and 1310 lymphoblast cell lines were used as representative for the two conditions. Two lymphoblast lines from Fanconi s anemia patients group A and C FA-A and FA-C respectively along with their isogenic corrected counterparts FA-A-cor and FA-C-cor were also used. Cell lines were evaluated for their proliferative ability vitality and apoptotic susceptibility upon microgravity exposure in comparison with unexposed cells. Different parameters correlated to energy metabolism glucose consumption mitochondrial membrane potential MMP intracellular ATP content red-ox balance and ability of the cells to repair the DNA damage product 8-OHdG induced by the treatment of the cells with 20 mM KBrO3 were also evaluated. Results Transformed Jurkat and 1310 cells appear resistant to the microgravitational challenge. On the contrary normal LB and HSC93 cells display increased apoptotic susceptibility shortage of energy storages and reduced ability to cope with oxidative stress. FA-A and FA-C cells appear resistant to microgravity exposure analogously to transformed cells. FA corrected cells did shown intermediate sensitivity to microgravity exposure suggesting that genetic correction does not completely

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