TAILIEUCHUNG - Báo cáo khoa học: Structural characterization of L-glutamate oxidase from Streptomyces sp. X-119-6

l-Glutamate oxidase (LGOX) from Streptomycessp. X-119-6, which cata-lyzes the oxidative deamination of l-glutamate, has attracted increasing attention as a component of amperometricl-glutamate sensors used in the food industry and clinical biochemistry. The precursor of LGOX, which has a homodimeric structure, is less active than the mature enzyme with an a2b2c2 structure; | Structural characterization of L-glutamate oxidase from Streptomyces sp. X-119-6 Jiro Arima1 2 z Chiduko Sasaki3 z Chika Sakaguchi1 Hiroshi Mizuno1 Takashi Tamura1 Akiko Kashima3 Hitoshi Kusakabe4 Shigetoshi Sugio3 and Kenji Inagaki1 1 Department of BiofunctionalChemistry Graduate Schoolof NaturalScience and Technology Okayama University Japan 2 Department of Agricultural Biological and EnvironmentalSciences Faculty of Agriculture Tottori University Japan 3 Innovation Center Yokohama Center Mitsubishi ChemicalCorporation Aoba-ku Yokohama Japan 4 Enzyme Sensor Co. Ltd Liaison Center 311 University of Tsukuba Japan Keywords L-amino acid oxidase L-glutamate oxidase Streptomyces substrate specificity X-ray crystallographic structure Correspondence K. Inagaki Department of Biofunctional Chemistry Graduate School of Natural Science and Technology Okayama University Okayama-shi Okayama 700-8530 Japan Fax 81 86 251 8299 Tel 81 86 251 8299 E-mail kinagaki@ S. Sugio Innovation Center Yokohama Center Mitsubishi Chemical Corporation Aoba-ku Yokohama 227-8502 Japan Fax 81 45 963 4206 Tel 81 45 963 3663 E-mail These authors contributed equally to this work Database Coordinate and structure factors of the mature L-glutamate oxidase have been deposited in the Protein Data Bank at the Research Collaboratory for Structural Bioinformatics under code 2E1M L-Glutamate oxidase LGOX from Streptomyces sp. X-119-6 which catalyzes the oxidative deamination of L-glutamate has attracted increasing attention as a component of amperometric L-glutamate sensors used in the food industry and clinical biochemistry. The precursor of LGOX which has a homodimeric structure is less active than the mature enzyme with an M2P2Y2 structure enzymatic proteolysis of the precursor forms the stable mature enzyme. We solved the crystal structure of mature LGOX using molecular replacement with a structurally homologous model of L-amino acid oxidase LAAO from .

• Báo cáo khoa học
• medical report
• Scientific reports
• scientific research
• l Glutamate oxidase (LGOX) from Streptomycessp
• X 119 6
• which cata lyzes the oxidative deamination of l glutamate
• has attracted increasing attention as a component of amperometricl glutamate sensors used in the food industry and clinical biochemistry
• The precursor of LGOX
• which has a homodimeric structure
• is less active than the mature enzyme with an a2b2c2 structure;
• thesis report
• medical knowledge
• medical research
• analysis of cytoplasmic
• Nobel Prizes
• health technologies
• medical ebook
• medical document
• medical specific study
• tumor cells
• chemical reaction
• Crystal structure
• medical analysis
• biochemical studies
• intermediate cells
• cell proliferation
• cytoplasm