TAILIEUCHUNG - Báo cáo lâm nghiệp: "Salicylic acid up-regulates the expression of chloroplastic Cu, Zn-superoxide dismutase in needles of maritime pine (Pinus pinaster Ait.)"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí lâm nghiệp đề tài: Salicylic acid up-regulates the expression of chloroplastic Cu, Zn-superoxide dismutase in needles of maritime pine (Pinus pinaster Ait.). | Ann. For. Sci. 61 2004 847-850 INRA EDP Sciences 2005 DOI forest 2004083 847 Note Salicylic acid up-regulates the expression of chloroplastic Cu Zn-superoxide dismutase in needles of maritime pine Pinus pinaster Ait. Herlânder Azevedo Teresa Lino-Neto Rui M. Tavares Grupo de Bioquímica e Fisiologia Molecular de Plantas Centro de Biologia Departamento de Biologia Universidade do Minho Campus de Gualtar 4710-057 Braga Portugal Received 7 May 2003 accepted 27 April 2004 Abstract - Several studies have supported a major role of salicylic acid in modulating plant response against abiotic and biotic stresses by induction antioxidant capacity. In this work a full-length cDNA encoding a Cu Zn - superoxide dismutase was isolated by screening a Pinus pinaster needle cDNA library. The predicted protein of 215 amino acid residues has a molecular mass of kDa and exhibits a N-terminal transit peptide which putatively targets the protein to the chloroplast. Treatment of pine seedlings with salicylic acid resulted in the increase of chloroplastic Cu Zn superoxide dismutase transcript levels in needles suggesting a role of this isoform in salicylic acid-mediated H2O2 increase in chloroplasts. Pinus pinaster salicylic acid chloroplastic Cu Zn-SOD Résumé - L acide salicylique induit une augmentation de l expression de la Cu Zn-superoxide dismutase du chloroplaste d aiguille de pin maritime Pinus pinaster Ait. . Plusieurs études suggèrent le rôle prédominant de l acide salicylique dans la modulation de la réponse des plantes aux stress abiotiques et biotiques par induction de la capacité antioxidante. Dans ce travail un ADNc pleine longueur codant pour une Cu Zn-superoxide dismutase a été isolé par criblage d une banque d ADNc d aiguille de Pinus pinaster. La protéine prédite de 215 acides aminés possède une masse moléculaire de 22 1 kDa et présente en N-terminal un peptide signale potentiel d adressage vers chloroplaste. Le traitement de jeunes plantules de pin par l .

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