TAILIEUCHUNG - Báo cáo lâm nghiệp: "Development and characteristics of microsatellite markers for sugi (Cryptomeria japonica D. Don) derived from microsatellite-enriched libraries"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí lâm nghiệp đề tài: Development and characteristics of microsatellite markers for sugi (Cryptomeria japonica D. Don) derived from microsatellite-enriched libraries. | Ann. For. Sci. 61 2004 569-575 INRA EDP Sciences 2004 DOI forest 2004052 569 Original article Development and characteristics of microsatellite markers for sugi Cryptomeria japonica D. Don derived from microsatellite-enriched libraries Naoki TANIa Tomokazu TAKAHASHIb Tokuko UJINO-IHARAa Hiroyoshi IWATAa c Kensuke YOSHIMURAa Yoshihiko TSUMURAa a Department of Forest Genetics Forestry and Forest Products Research Institute Matsunosato Tsukuba Ibaraki 305-8687 Japan b Graduate School of Science and Technology Niigata University Ikarashi Niigata 950-2181 Japan c Present address National Agricultural Research Center Kannondai Tsukuba Ibaraki 305-8666 Japan Received 11 July 2003 accepted 18 March 2004 Abstract - We have developed a series of microsatellite markers for C. japonica. First DNA fragments including microsatellite sequences were isolated from two GA-enriched genomic libraries using magnetic beads. After eliminating redundant clones and clones in which the tandem repeats were located too close to the cloning site to allow primers to be constructed the remaining sequences could be examined for their suitability for primer design. Primer sets were designed from each conserved sequence flanking the microsatellites. We found 1 479 unique sequences in the enriched genomic libraries of which 962 contained a tandem repeat motif and we have been able to design 196 primer pairs using these sequences to date. The potential of these primers to amplify single fragment and the polymorphism of the sequences they amplify were investigated using a panel of 28 plus trees selected from Cryptomeria plantations covering the wide distributional range of the species in Japan. Forty-two of the microsatellite markers displayed a polymorphic nature throughout this panel of 28 DNA samples. The polymorphic information coefficients PICs ranged from to . There was a significant correlation between the number of repeats and the size of the PICs according to Kendall s T .

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