TAILIEUCHUNG - báo cáo khoa học: "Production of viable hybrids in salmonids by triploidization"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Production of viable hybrids in salmonids by triploidization | Génét. Séỉ. Evol. 1983 15 4 519-532 Production of viable hybrids in salmonids by triploldization B. CHEVASSUS R. GUYOMARD D. CHOURROUT and Edwige QUILLET with the technical assistance of G. Burger and A. Devaux . Laboratoire de Physiologic des Poissons F 78350 Jouy-en-Josas . Centre Océanologie de Bretagne F 29273 Brest Cedex Summary Rainbow trout eggs were fertilized with milt collected from various salmonids brown trout Salmo trutta brook trout Salvelinus fontinalis coho salmon Oncorhynchus kisutch and heat-shocked few minutes later in order to prevent the second polar body extrusion. This operation resulted in high survival rates after 161 days respectively p. 100 p. 100 and p. 100 of the diploid rainbow trout control basis 100 p. 100 the triploid hybrid constitution of these fish was ascertained by karyological and biochemical investigations. These data contrast with the bad yields of the corresponding diploid hybridizations no heat shock respectively 0 p. 100 p. 100 and 0 p. 100. Such triploid hybrid salmonids that are easy to produce could be sterile and consequently of a great interest in aquaculture where fish reproduction often makes the profitability decrease. Key words Hybrids triploids salmonids. Resume Production d hybrides viables chez les salmonidés par triploidisation Des ovules de truite arc-en-ciel Salmo gairdneri ont été inséminés par du sperme de divers salmonidés truite fario Salmo trutta omble de fontaine Salvelinus fontinalis saumon coho Oncorhynchus kisutch puis soumis ậ un choc thermique chaud induisant la retention du 2e globule polaire. Des individus viables ont été obtenus à un taux élevé les taux de survie à 161 jours rapportés au témoin diploide arc-en-ciel s elevent respecti-vement à 49 2 p. 100 66 2 p. 100 et 12 5 p. 100. Les donnees caryologiques et biochimiques indiquent que tons ces animaux sont des hybrides triploides. Par con ti e les memes croisements non soumis à un choc chaud ne produisent

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