TAILIEUCHUNG - Báo cáo khoa hoc:"A higher resolution radiation hybrid map of bovine chromosome 13"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: A higher resolution radiation hybrid map of bovine chromosome 13 | Genet. Sel. Evol. 34 2002 255-267 255 INRA EDP Sciences 2002 DOI gse 2002007 Original article A higher resolution radiation hybrid map of bovine chromosome 13 Jorg ScHLAPFERa Nasikhat STAHLBERGER-SAITBEKOVAa Sergio CoMiNCiNib Claude GAiLLARDa David Hillsc Rudolf K. MEYERd John L. WiLLiAMSc Jim E. WoMACKe Andreas ZuRBRiGGENd Gaudenz DoLFa a institute of Animal Genetics Nutrition and Housing Faculty of Veterinary Medicine University of Berne Bremgartenstrasse 109 a 3012 Berne Switzerland b Department of Genetics and Microbiology University of Pavia Via Ferrata 1 271000 Pavia italy c Division of Genomics and Bioinformatics Roslin institute Edinburgh Roslin Midlothian EH25 9PS UK d institute of Animal Neurology Faculty of Veterinary Medicine University of Berne Bremgartenstrasse 109 a 3012 Berne Switzerland e Department of Veterinary Pathobiology College of Veterinary Medicine Texas A M University College Station Texas 77843 USA Received 25 June 2001 accepted 7 December 2001 Abstract - in this paper we present a radiation hybrid framework map of BTA13 composed of nine microsatellite loci six genes and one EST. The map has been developed using a recently constructed 12 000 rad bovine-hamster whole-genome radiation hybrid panel. Moreover we present a comprehensive map of BTA13 comprising 72 loci of which 45 are microsatellites 20 are genes and seven are ESTs. The map has an estimated length of cR12 00Q. The proposed order is in general agreement with published maps of BTA13. Our results only partially support previously published information of five blocks of conserved gene order between cattle and man. We found no evidence for the existence of an HSA20 homologous segment of coding DNA on BTA13 located centromeric of a confirmed HSA10 homologous region. The present map increases the marker density and the marker resolution on BTA13 and enables further insight into the evolutionary development of the chromosome as compared to man. bovine chromosome 13 .

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