TAILIEUCHUNG - Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR

Birth control pills: Using birth control pills lowers the risk of endometrial cancer. The risk is lowest in women who take the pill for a long time. And this protection goes on for at least 10 years after a woman stops taking this form of birth control. But you need to look at all of the pros and cons when choosing a birth control method -- endometrial cancer risk is only one factor to think about. It's a good idea to talk to your doctor about the different methods of birth control to find the one that is best for. | Watanabe et al. Cancer Cell International 2010 10 2 http content 10 1 2 CANCER CELL INTERNATIONAL PRIMARY RESEARCH Open Access Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR 1 1 1 1 2 3 Takashi Watanabe Tomohiro Miura Yusuke Degawa Yuna Fujita Masaaki Inoue Makoto Kawaguchi Chie Furihata1 Abstract Background Lung cancers are the most common type of human malignancy and are intractable. Lung cancers are generally classified into four histopathological subtypes adenocarcinoma AD squamous cell carcinoma SQ large cell carcinoma LC and small cell carcinoma SC . Molecular biological characterization of these subtypes has been performed mainly using DNA microarrays. In this study we compared the gene expression profiles of these four subtypes using twelve human lung cancer cell lines and the more reliable quantitative real-time PCR qPCR . Results We selected 100 genes from public DNA microarray data and examined them by DNA microarray analysis in eight test cell lines A549 ABC-1 EBC-1 LK-2 LU65 LU99 STC 1 RERF-LC-MA and a normal control lung cell line MRC-9 . From this we extracted 19 candidate genes. We quantified the expression of the 19 genes and a housekeeping gene GAPDH with qPCR using the same eight cell lines plus four additional validation lung cancer cell lines RERF-LC-MS LC-1 sq 86-2 and MS-1-L . Finally we characterized the four subtypes of lung cancer cell lines using principal component analysis PCA of gene expression profiling for 12 of the 19 genes AMY2A CDH1 FOXG1 IGSF3 ISL1 MALL PLAU RAB25 S100P SLCO4A1 STMN1 and TGM2 . The combined PcA and gene pathway analyses suggested that these genes were related to cell adhesion growth and invasion. S100P in AD cells and CDH1 in AD and SQ cells were identified as candidate markers of these lung cancer subtypes based on their upregulation and the results of PCA analysis. Immunohistochemistry for S100P .

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