TAILIEUCHUNG - Báo cáo sinh học: " Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Effects of transplantation with bone marrowderived mesenchymal stem cells modified by Survivin on experimental stroke in rats | Liu et al. Journal of Translational Medicine 2011 9 105 http content 9 1 105 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Effects of transplantation with bone marrow-derived mesenchymal stem cells modified by Survivin on experimental stroke in rats 1 3 4 1 1 1 1 Nan Liu Yixian Zhang Lin Fan Mingzhou Yuan Houwei Du Ronghua Cheng Deshan Liu and Feifei Lin Abstract Background This study was performed to determine whether injury induced by cerebral ischemia could be further improved by transplantation with bone marrow-derived mesenchymal stem cells MSCs modified by Survivin SVV . Methods MSCs derived from bone marrow of male Sprague-Dawley rats were infected by the self-inactive lentiviral vector GCFU carrying green fluorescent protein GFP gene and SVV recombinant vector GCFU-SVV . In vitro vascular endothelial growth factor VEGF and basic fibroblast growth factor bFGF were detected in infected MSCs supernatants under hypoxic conditions by ELSIA. In vivo experiments consisted of three groups one receiving intravenous injection of 500 gl of phosphate-buffered saline PBS without cells control group and two groups administered the same volume solution with either three million GFP-MSCs group GFP or SVV GFP-MSCs group SVV . All animals were submitted to 2-hour middle cerebral artery occlusion MCAO and then reperfusion. Differentiation and survival of the transplanted MSCs were determined by confocal microscope. Western blot was used to detect the expression of VEGF and bFGF in ischemic tissue. A 2 3 5-triphenyltetrazolium chloride TTC staining was used to assess the infarct volume. Evaluation of neurological function was performed using a modified Neurological Severity Score mNSS . Results In vitro modification with SVV further increased secretion of VEGF and bFGF under hypoxic condition. In vivo only very few transplantated cells co-expressed GFP and NeuN. The survival transplanted cells in the group SVV was at 4 days

TÀI LIỆU LIÊN QUAN
TAILIEUCHUNG - Chia sẻ tài liệu không giới hạn
Địa chỉ : 444 Hoang Hoa Tham, Hanoi, Viet Nam
Website : tailieuchung.com
Email : tailieuchung20@gmail.com
Tailieuchung.com là thư viện tài liệu trực tuyến, nơi chia sẽ trao đổi hàng triệu tài liệu như luận văn đồ án, sách, giáo trình, đề thi.
Chúng tôi không chịu trách nhiệm liên quan đến các vấn đề bản quyền nội dung tài liệu được thành viên tự nguyện đăng tải lên, nếu phát hiện thấy tài liệu xấu hoặc tài liệu có bản quyền xin hãy email cho chúng tôi.
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.