TAILIEUCHUNG - Báo cáo lâm nghiệp: "Changes in phenolic acids and stilbenes induced in embryogenic cell cultures of Norway spruce by two fractions of Sirococcus strobilinus mycelia"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí lâm nghiệp đề tài: Changes in phenolic acids and stilbenes induced in embryogenic cell cultures of Norway spruce by two fractions of Sirococcus strobilinus mycelia. | JOURNAL OF FOREST SCIENCE 57 2011 1 1-7 Changes in phenolic acids and stilbenes induced in embryogenic cell cultures of Norway spruce by two fractions of Sirococcus strobilinus mycelia J. Malá1 M. Hrubcová2 P. Máchová1 H. Cvrcková1 O. Martincová2 M. Cvikrová2 forestry and Game Management Research Institute Jtloviste Czech Republic 2Institute of Experimental Botany Academy of Sciences of the Czech Republic Prague Czech Republic ABSTRACT We examined defence responses in embryogenic cell suspension cultures of Norway spruce Picea abies L. Karst elicited by intracellular protein and cell wall fractions PF and WF respectively prepared from mycelia of the fungus Sirococcus strobilinus Preuss focusing on changes in soluble and cell wall-bound phenolic and stilbene concentrations. Treatment with both preparations induced an increase in the total contents of phenolic acids in Norway spruce cells and variations in the levels of stilbene glycosides. More rapid and intense induction of defence response was observed in cells after WF application. The contents of soluble phenolic acids especially benzoic acid derivatives and cell wall-bound phenolic acids especially ferulic acid started to increase relative to controls within 4 h after the addition of the WF preparation and remained high in elicited cells for 8-12 h. A moderate increase in phenolic acids in cells exposed to the PF preparation was observed within 8 h after application. However after 24 h of WF treatment a decline of total phenolics was observed while in PF elicited Norway spruce cells the phenolic content continued to increase. Significantly decreased concentrations of stilbene glycosides isorhapontin astringin and piceid were determined in PF and WF treated Norway spruce cell cultures. The total content of stilbene glycosides decreased within 8 h after WF application to 68 of the amount determined in the control and within 12 h to 73 of the control in PF-treated cells. These results demonstrate that both PF and

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