TAILIEUCHUNG - Báo cáo sinh học: " Double suicide genes driven by kinase domain insert containing receptor promoter selectively kill human lung cancer cells"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Double suicide genes driven by kinase domain insert containing receptor promoter selectively kill human lung cancer cells | Ma et al. Genetic Vaccines and Therapy 2011 9 6 http content 9 1 6 z GENETIC VACCINES AND THERAPY RESEARCH Open Access Double suicide genes driven by kinase domain insert containing receptor promoter selectively kill human lung cancer cells 11 11 2 3 2 X 1 z-1 2 Junrong Ma Mi Li Longyong Mei Qinghua Zhou Lunxu Liu Xijie Yu Guowei Che Abstract Background To investigate the selective killing efficacy of the double suicide genes driven by KDR promoter. Materials and methods A double suicide gene system with the KDR promoter pcDNA3-KDRp-CDglyTK was constructed and transfected into lung cancer cell lines L9981 and NL9980 and human hepatocellular carcinoma cell line HepG2. The efficiency and specificity of the double suicide gene system were assayed by in vitro cellular proliferation and apoptosis as well as in vivo xenograft studies. Results The transgenic CD and TK genes were only expressed in L9981 and NL9980 but not in HepG2 cells. Pretreating transfected cells with 5-Fc and GCV significantly reduced proliferation enhanced apoptosis in L9981 and NL9980 but not in HepG2 cells. The tumor formed by L9981 and NL9980 cells with the double suicide gene system was much smaller in vivo. Conclusion Tumor targeted expression of CDglyTK gene driven by KDR promotor represents a novel strategy for effective gene therapy of tumor with intrinsic KDR. Background Tumor-specific targeting gene therapy is a widely used anti-tumor method. Regulated expression of a suicide gene with the promoters can primarily destroy tumor cells and leave the surrounding tissues undamaged. The a-fetoprotein promoter AFP is such a representative to activate an exogenous gene expression specifically in hepatocellular carcinoma HCC and has been applied to targeted gene therapy for HCC 1 . Nishino et al reported an approach to selectively kill c-Myc-expressing lung cancer cells by fusing the c-Myc gene promoter with TK gene 2 . Previous studies have shown that the KDR gene is .

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