TAILIEUCHUNG - Báo cáo sinh học: "Human cytomegalovirus plasmid-based amplicon vector system for gene therapy"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Human cytomegalovirus plasmid-based amplicon vector system for gene therapy | Genetic Vaccines and Therapy BioMed Central Research Open Access Human cytomegalovirus plasmid-based amplicon vector system for gene therapy Kutubuddin Mahmood Mark N Prichard Gregory M Duke George W Kemble and Richard R Spaete Address MedImmune Vaccines Inc. 297 North Bernardo Avenue Mountain View CA 94043 USA Email Kutubuddin Mahmood - mahmoodk@ Mark N Prichard - mprichard@ Gregory M Duke - dukeg@ George W Kemble - kembleg@ Richard R Spaete - spaeter@ Corresponding author Published 26 January 2005 Received 28 August 2004 Accepted 26 January 2005 Genetic Vaccines and Therapy 2005 3 1 doi 1479-0556-3-1 This article is available from http content 3 1 1 2005 Mahmood et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We have constructed and evaluated the utility of a helper-dependent virus vector system that is derived from Human Cytomegalovirus HCMV . This vector is based on the herpes simplex virus HSV amplicon system and contains the HCMV orthologs of the two cis-acting functions required for replication and packaging of HSV genomes the complex HCMV viral DNA replication origin oriLyt and the cleavage packaging signal the a sequence . The HCMV amplicon vector replicated independently and was packaged into infectious virions in the presence of helper virus. This vector is capable of delivering and expressing foreign genes in infected cells including progenitor cells such as human CD34 cells. Packaged defective viral genomes were passaged serially in fibroblasts and could be detected at passage 3 however the copy number appeared to diminish upon serial passage. The HCMV amplicon offers an alternative vector strategy useful

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