TAILIEUCHUNG - Báo cáo sinh học: "Skipping the co-expression problem: the new 2A "CHYSEL" technology"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Skipping the co-expression problem: the new 2A "CHYSEL" technology | Genetic Vaccines and Therapy BioMed Central Commentary Open Access Skipping the co-expression problem the new 2A CHYSEL technology Pablo de Felipe Address Centre for Biomolecular Sciences School of Biology Biomolecular Sciences Building University of St. Andrews North Haugh St. Andrews KY16 9ST Scotland UK Email Pablo de Felipe - pdf@ Corresponding author Published 13 September 2004 Received 30 June 2004 Accepted 13 September 2004 Genetic Vaccines and Therapy 2004 2 1 3 doi 1479-0556-2-13 This article is available from http content 2 1 13 2004 de Felipe licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The rapid progress in the field of genomics is increasing our knowledge of multi-gene diseases. However any realistic hope of gene therapy treatment for those diseases needs first to address the problem of co-ordinately co-expressing several transgenes. Currently the use of internal ribosomal entry sites IRESs is the strategy chosen by many researchers to ensure co-expression. The large sizes of the IRESs kb and the difficulties of ensuring a well-balanced co-expression have prompted several researchers to imitate a co-expression strategy used by many viruses to express several proteins as a polyprotein. A small peptide of 18 amino acids 2A from the foot-and-mouth disease virus FMDV is being used to avoid the need of proteinases to process the polyprotein. FMDV 2A is introduced as a linker between two proteins to allow autonomous intra-ribosomal self-processing of polyproteins. Recent reports have shown that this sequence is compatible with different sub-cellular targeting signals and can be used to co-express up to four proteins from a single retroviral vector. This .

• Báo cáo khoa học
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học