TAILIEUCHUNG - Báo cáo sinh học: "RNAi and the shape of things to come"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: RNAi and the shape of things to come. | J. Biol. Journal of Biology BioMed Central Research news RNAi and the shape of things to come Jonathan B Weitzman Published 4 November 2003 Journal of Biology 2003 2 23 The electronic version of this article is the complete one and can be found online at http content 2 4 23 2003 BioMed Central Ltd A large-scale screen in Drosophila cells has shown how RNA interference can provide insights into the pathways controlling cell morphology. When thinking of genetic screens in Drosophila the Nobel-prize-winning screens for embryonic developmental phenotypes spring to mind. Loss-of-function mutational analysis has proved to be a powerful approach for dissecting complex processes such as morphogenesis and wing development. But historically the fruitfly community has focused much less on the study of cells in culture. All that may now be about to change. In this issue of Journal of Biology 1 Amy Kiger and colleagues describe the results of a The bottom line Kiger and colleagues have demonstrated that RNA-interference RNAi technology can be used in automated high-throughput screens in Drosophila cells in culture. Systematic loss-of-function analysis of nearly 1 000 predicted cellshape regulators has identified sets of genes involved in cellular morphogenesis. Standardized phenotypic annotation defines an RNAi signature for each gene that can be used to predict the function of unknown genes. This proof of principle study provides the first steps in a genomewide analysis of cell morphology. powerful strategy for conducting high-throughput systematic loss-of-function screens in Drosophila tissue-culture cells see The bottom line box for a summary of the work . Picking apart signaling pathways The study was led by Norbert Perri-mon a Howard Hughes Medical Institute investigator who runs a Drosophila laboratory at Harvard Medical School in Boston USA. Perrimon has focused his lab on developing large-scale genetic screens to dissect signal transduction pathways by .

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