TAILIEUCHUNG - Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid

Carbohydrates of laminin, a family of large multidomain glycoproteins, have been implicated in various cellular activities including maintaining the protein structure, its function and also basement membrane integrity. Dur-ing the course of our investigation | 1FEBS Journal Structural characterization of N-linked oligosaccharides of laminin from rat kidney changes during diabetes and modulation by dietary fiber and butyric acid Adishesha Puneeth Kumar Chilkunda D. Nandini and Paramahans V. Salimath Department of Biochemistry and Nutrition CentralFood TechnologicalResearch Institute Mysore India Keywords diabetes kidney laminin MALDI-TOF oligosaccharides Correspondence P. V. Salimath Dept. of Biochemistry and Nutrition CentralFood Technological Research Institute Mysore - 570 020 Karnataka India Fax 91 821 251 7233 Tel 91 821 251 4876 E-mail salimath1954@ Received 14 June 2010 revised 28 September 2010 accepted 26 October 2010 doi Carbohydrates of laminin a family of large multidomain glycoproteins have been implicated in various cellular activities including maintaining the protein structure its function and also basement membrane integrity. During the course of our investigation we observed that purified laminin from kidneys of control diabetic and dietary fiber- and butyric acid-treated diabetic rats showed differences in binding to extracellular matrix components. This prompted us to determine whether there are structural changes in laminin oligosaccharides. In this study we have characterized a few major N-linked oligosaccharides isolated from purified laminin in various experimental groups viz. normal diabetic and diabetic rats fed with dietary fiber and butyric acid. Sugar composition as identified by GLC revealed the presence of mannose galactose and N-acetylglucosamine. In order to study fine structures of the oligosaccharides N-linked oligosaccharides of laminin were released by Peptide-N-glycosidase F digestion end-labeled with 2-anthranilic acid and fractionated by lectin affinity chromatography. Furthermore structural elucidation carried out by MALDI-TOF MS MS analysis showed variations in the oligosaccharide sequence of laminin during diabetes which were altered by .

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