TAILIEUCHUNG - Báo cáo khoa học: Copines-1, -2, -3, -6 and -7 show different calcium-dependent intracellular membrane translocation and targeting

The copines are a family of C2- and von Willebrand factor A-domain-con-taining proteins that have been proposed to respond to increases in intra-cellular calcium by translocating to the plasma membrane. The copines have been reported to interact with a range of cell signalling and cytoskele-tal proteins, which may therefore be targeted to the membrane following increases in cellular calcium. | IFEBS Journal Copines-1 -2 -3 -6 and -7 show different calcium-dependent intracellular membrane translocation and targeting Pavel V. Perestenko Amy M. Pooler Maryam Noorbakhshniaf Adrian GrayỊ Charlotte Bauccio and Robert Andrew Jeffrey McIlhinney MedicalResearch CouncilAnatomicalNeuropharmacology Unit Oxford UK Keywords C2-domains copines HEK-293 intracellular calcium vWA-domain Correspondence P. V. Perestenko MedicalResearch Council Anatomical Neuropharmacology Unit Mansfield Road Oxford OX1 3TH UK Fax 44 1865 271647 Tel 44 1865 271866 E-mail Present addresses Medical Research Council Centre for Neurodegeneration Research Institute of Psychiatry Department of Neuroscience King s College London UK fDepartment of Biology Faculty of Science Isfahan University Iran ỊSir William Dunn Schoolof Pathology Oxford UK Trinity College Oxford UK Received 21 June 2010 revised 15 October 2010 accepted 22 October 2010 doi The copines are a family of C2- and von Willebrand factor A-domain-con-taining proteins that have been proposed to respond to increases in intracellular calcium by translocating to the plasma membrane. The copines have been reported to interact with a range of cell signalling and cytoskele-tal proteins which may therefore be targeted to the membrane following increases in cellular calcium. However neither the function of the copines nor their actual movement to the plasma membrane has been fully established in mammalian cells. Here we show that copines-1 -2 -3 -6 and -7 respond differently to a methacholine-evoked intracellular increase in calcium in human embryonic kidney cell line-293 cells and that their membrane association requires different levels of intracellular calcium. We demonstrate that two of these copines associate with different intracellular vesicles following calcium entry into cells and identify a novel conserved amino acid sequence that is required for their membrane .

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