TAILIEUCHUNG - Báo cáo khoa học: Evolution of the teleostean zona pellucida gene inferred from the egg envelope protein genes of the Japanese eel, Anguilla japonica

A fish egg envelope is composed of several glycoproteins, called zona pellu-cida (ZP) proteins, which are conserved among vertebrate species. Euteleost fishes synthesize ZP proteins in the liver, while otocephalans synthesize them in the growing oocyte. We investigated ZP proteins of the Japanese eel, Anguilla japonica, belonging to Elopomorpha, which diverged earlier than Euteleostei and Otocephala. | Evolution of the teleostean zona pellucida gene inferred from the egg envelope protein genes of the Japanese eel Anguilla japonica Kaori Sano1 Mari Kawaguchi2 Masayuki Yoshikawa3 Ichiro luchi4 and Shigeki Yasumasu4 1 Graduate Program of BiologicalScience Graduate Schoolof Science and Technology Sophia University Tokyo Japan 2 Atmosphere and Ocean Research Institute The University of Tokyo Japan 3 Suruga-Bay Deep Sea Water Aquaculture Research Center Shizuoka PrefecturalResearch Institute of Fishery Japan 4 Department of Materials and Life Sciences Faculty of Science and Technology Sophia University Tokyo Japan Keywords egg envelope expression profile Japanese eel molecular evolution ZP domain Correspondence S. Yasumasu Department of Materials and Life Sciences Faculty of Science and Technology Sophia University 7-1 Kioi-cho Chiyoda-ku Tokyo 102-8554 Japan Fax 81 3 3238 3393 Tel 81 3 3238 3270 E-mail s-yasuma@ Received 12 July 2010 revised 30 August 2010 accepted 9 September 2010 doi A fish egg envelope is composed of several glycoproteins called zona pellu-cida ZP proteins which are conserved among vertebrate species. Euteleost fishes synthesize ZP proteins in the liver while otocephalans synthesize them in the growing oocyte. We investigated ZP proteins of the Japanese eel Anguilla japonica belonging to Elopomorpha which diverged earlier than Euteleostei and Otocephala. Five major components of the egg envelope were purified and their partial amino acid sequences were determined by sequencing. cDNA cloning revealed that the eel egg envelope was composed of four ZPC homologues and one ZPB homologue. Four of the five eel ZP eZP proteins possessed a transmembrane domain which is not found in the ZP proteins of Euteleostei and Otocephala that diverged later but is found in most other vertebrate ZP proteins. This result suggests that fish ZP proteins originally possessed a transmembrane domain and lost it during .

TỪ KHÓA LIÊN QUAN
TÀI LIỆU MỚI ĐĂNG
9    211    0    29-03-2024
46    179    0    29-03-2024
8    163    0    29-03-2024
20    189    2    29-03-2024
37    146    0    29-03-2024
10    112    0    29-03-2024
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.