TAILIEUCHUNG - Báo cáo khoa học: Exogenous oxidative stress induces Ca2+ release in the yeast Saccharomyces cerevisiae

The Ca 2+ -dependent response to oxidative stress caused by H2O2 ortert-butylhydroperoxide (tBOOH) was investigated inSaccharomyces cerevisiae cells expressing transgenic cytosolic aequorin, a Ca 2+ -dependent photopro-tein. Both H2O2 and tBOOH induced an immediate and short-duration cytosolic Ca 2+ increase that depended on the concentration of the stres-sors. | ỊFEBS Journal Exogenous oxidative stress induces Ca2 release in the yeast Saccharomyces cerevisiae Claudia-Valentina Popa loana Dumitru Lavinia L. Ruta Andrei F. Danet and Ileana C. Farcasanu Faculty of Chemistry University of Bucharest Romania Keywords aequorin cytosolic calcium oxidative stress Saccharomyces cerevisiae yeast Correspondence I. C. Farcasanu University of Bucharest Faculty of Chemistry Sos. Panduri 90-92 Bucharest Romania Fax 40 021 410 01 40 Tel 40 721067169 E-mail Received 16 April 2010 revised 2 July 2010 accepted 28 July 2010 doi The Ca2 -dependent response to oxidative stress caused by H2O2 or tertbutylhydroperoxide tBOOH was investigated in Saccharomyces cerevisiae cells expressing transgenic cytosolic aequorin a Ca2 -dependent photoprotein. Both H2O2 and tBOOH induced an immediate and short-duration cytosolic Ca2 increase that depended on the concentration of the stressors. Sublethal doses of H2O2 induced Ca2 entry into the cytosol from both extracellular and vacuolar sources whereas lethal H2O2 shock mobilized predominantly the vacuolar Ca2 . Sublethal and lethal tBOOH shocks induced mainly the influx of external Ca2 accompanied by a more modest vacuolar contribution. Ca2 transport across the plasma membrane did not necessarily involve the activity of the Cch1p Mid1p channel whereas the release of vacuolar Ca2 into the cytosol required the vacuolar channel Yvc1p. In mutants lacking the Ca2 transporters H2O2 or tBOOH sensitivity correlated with cytosolic Ca2 overload. Thus it appears that under H2O2-induced or tBOOH-induced oxidative stress Ca2 mediates the cytotoxic effect of the stressors and not the adaptation process. Introduction Eukaryotic cells from yeast to mammals respond and adapt to environmental stress by evolutionarily conserved multicomponent endogenous systems that utilize a network of signal transduction pathways to regulate the adaptive and protective phenotype. .

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