TAILIEUCHUNG - báo cáo khoa học: " UV-B-induced signaling events leading to enhanced-production of catharanthine in Catharanthus roseus cell suspension cultures"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: UV-B-induced signaling events leading to enhanced-production of catharanthine in Catharanthus roseus cell suspension cultures | BMC Plant Biology BioMed Central Research article Open Access UV-B-induced signaling events leading to enhanced-production of catharanthine in Catharanthus roseus cell suspension cultures Shilpa Ramani and Jayabaskaran Chelliah Address Department of Biochemistry Indian Institute of Science Bangalore 560012 India Email Shilpa Ramani - shilpasuhas@ Jayabaskaran Chelliah - cjb@ Corresponding author Published 7 November 2007 Received 13 November 2006 DAAZ-- AA-7 J_ .IA I IOZ IATI A 1 I Accepted 7 November 2007 BMC Plant Biology 2007 7 61 doi l47l-2229-7-6l This article is available from http l47l-2229 7 6l 2007 Ramani and Chelliah licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Elicitations are considered to be an important strategy towards improved in vitro production of secondary metabolites. In cell cultures biotic and abiotic elicitors have effectively stimulated the production of plant secondary metabolites. However molecular basis of elicitor-signaling cascades leading to increased production of secondary metabolites of plant cell is largely unknown. Exposure of Catharanthus roseus cell suspension culture to low dose of UV-B irradiation was found to increase the amount of catharanthine and transcription of genes encoding tryptophan decarboxylase Tdc and strictosidine synthase Str . In the present study the signaling pathway mediating UV-B-induced catharanthine accumulation in C. roseus suspension cultures were investigated. Results Here we investigate whether cell surface receptors medium alkalinization Ca2 influx H2O2 CDPK and MAPK play required roles in UV-B signaling leading to enhanced production of catharanthine in C. roseus cell .

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