TAILIEUCHUNG - Báo cáo khoa học: A novel neuropeptide (FRamide) family identified by a peptidomic approach in Hydra magnipapillata

In the course of systematic identification of peptide signaling molecules combined with the expressed sequence tag database fromHydra, we have identified a novel neuropeptide family that consists of two members with FRamide at the C-terminus; FRamide-1 (IPTGTLIFRamide) and FR-amide-2 (APGSLLFRamide). | ễFEBS Journal A novel neuropeptide FRamide family identified by a peptidomic approach in Hydra magnipapillata Eisuke Hayakawa1 Toshio Takahashi2 Chiemi Nishimiya-Fujisawa1 and Toshitaka Fujisawa1 1 Department of DevelopmentalGenetics National institute of Genetics Mishima Shizuoka Japan 2 Suntory Institute for Bioorganic Research Shimamoto-cho Osaka Japan Keywords cnidaria FRamide Hydra muscle contraction neuropeptide Correspondence T. Fujisawa National institute of Genetics Yata 1 111 Mishima 411-8540 Japan Fax 81 55 981 6768 Tel 81 55 981 6765 E-mail tfujisaw@ Database The nucleotide sequence is available in the DDBJ database under the accession number AB266099 These authors contributed equally to this work Received 3 July 2007 revised 24 August 2007 accepted 28 August 2007 doi In the course of systematic identification of peptide signaling molecules combined with the expressed sequence tag database from Hydra we have identified a novel neuropeptide family that consists of two members with FRamide at the C-terminus FRamide-1 IPTGTLIFRamide and FR-amide-2 APGSLLFRamide . The precursor sequence deduced from cDNA contained a single copy each of FRamide-1 and FRamide-2 precursor sequences. Expression analysis by whole-mount in situ hybridization showed that the gene was expressed in a subpopulation of neurons that were distributed throughout the body from tentacles to basal disk. Double in situ hybridization analysis showed that the expressing cell population was further subdivided into one population consisting of neurons expressing both the FRamide and Hym176 neuropeptide genes and the other consisting of neurons expressing only the FRamide gene. FRamide-1 evoked elongation of the body column of epithelial Hydra that was composed of epithelial cells and gland cells but lacked all the cells in the interstitial stem cell lineage including neurons. In contrast FRamide-2 evoked body column contraction. These results suggest

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