TAILIEUCHUNG - Báo cáo khoa học: YSF – Young Scientist Forum

One ot the most representative (sub)tropical soil microbe,Chro-mobacterium violaceum, produces many important substances and has great potential for biotechnology and medicine. On the other hand, it can also act as an opportunistic human pathogen. The bacterium produces the lectin CV-IIL, that can help the adhesion bacterium to saccharide moieties at host cells¢ surface. | YSF - Young Scientist Forum ẶFEBS Journal YSF - Young Scientist Forum CV-IIL lectin another piece of the Chromobacterium violaceum puzzle L. Adamova1 J. Adam1 and M. Wimmerova1 2 National Centre for Biomolecular Research Kotlarska 2 Brno Czech Republic 2Department of Biochemistry Masaryk University Kotlarska 2 Brno Czech Republic One ot the most representative sub tropical soil microbe Chromobacterium violaceum produces many important substances and has great potential for biotechnology and medicine. On the other hand it can also act as an opportunistic human pathogen. The bacterium produces the lectin CV-IIL that can help the adhesion bacterium to saccharide moieties at host cells surface. This step is essential for enabling the colonization of host tissue by the bacteria in organism and therefore for starting the infection. Understanding the principle of the interaction can be helpful in designing anti-adhesion therapy. CV-IIL belongs to the PA-IIL lectin family which includes structurally similar lectins from several other pathogenic bacteria. These lectins have increased affinity for monosaccharides thanks to two calcium ions in the binding site that mediate the sugar-lec-tin interaction. The CV-IIL lectin has a comparable affinity to fucose and mannose the other lectins strongly prefer one or the other and therefore it is ideal for testing of specificity change by mutation. This work is focused on the important amino acid triad 22-23-24 which is suspected to play a decisive role in directing the specificity. Mutants of CV-IIL were prepared by in silico and in vitro directed mutagenesis method their binding properties were tested by surface plasmon resonance and isothermal titration calorimetry. Complemented with molecular docking a powerful molecular modeling method the results of the study offer important additional insight into the structural reasoning behind the sugar preference as well as provide another part of the wider picture of this important .

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