TAILIEUCHUNG - Báo cáo khoa học: cceleration of disulfide-coupled protein folding using glutathione derivatives

Silicateins are unique enzymes of sponges (phylum Porifera) that template and catalyze the polymerization of nanoscale silicate to siliceous skeletal elements. These multifunctional spicules are often elaborately shaped, with complex symmetries. | 1FEBS Journal Silintaphin-1 - interaction with silicatein during structure-guiding bio-silica formation Ute SchloBmacher1 Matthias Wiens1 Heinz C. Schroder1 Xiaohong Wang2 Klaus P. Jochum3 and Werner E. G. Muller1 1 Institute for PhysiologicalChemistry Johannes Gutenberg-University Medical School Mainz Germany 2 NationalResearch Center for Geoanalysis Beijing China 3 Max Planck Institute for Chemistry Mainz Germany Keywords Porifera silicatein silintaphin-1 sponges Suberites domuncula Correspondence W. E. G. Muller Institute for Physiological Chemistry Johannes Gutenberg-University MedicalSchool Duesbergweg 6 D-55099 Mainz Germany Fax 49 6131 39 25243 Tel 49 6131 39 25910 E-mail wmueller@ Received 18 December 2010 revised 15 January 2011 accepted 28 January 2011 doi Silicateins are unique enzymes of sponges phylum Porifera that template and catalyze the polymerization of nanoscale silicate to siliceous skeletal elements. These multifunctional spicules are often elaborately shaped with complex symmetries. They carry an axial proteinaceous filament consisting of silicatein and the scaffold protein silintaphin-1 which guides silica deposition and subsequent spicular morphogenesis. In vivo the synthesis of the axial filament very likely proceeds in three steps a assembly of silicatein monomers to form one pentamer b assembly of pentamers to form fractal-like structures and finally c assembly of fractal-like structures to form filaments. The present study was aimed at exploring the effect of selfassembled complexes of silicatein and silintaphin-1 on biosilica synthesis in vitro. Hence in a comparative approach recombinant silicatein and recombinant silintaphin-1 were used at different stoichiometric ratios to form axial filaments and to synthesize biosilica. Whereas recombinant sili-catein-a reaggregates to randomly organized structures coincubation of silicatein-a and silintaphin-1 molecular ratio 4 1 resulted in synthetic .

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