TAILIEUCHUNG - Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii "

Proteinases are involved in different crucial living processes such as digestion, germination, growth, development, metamorphism, fertilisation, blood clotting, infection diseases, cancer etc. Therefore their protein inhibitors (PPIs) – an effective regulation factors have been used in medicine for treatment of diseases and in agriculture to improve plant resistance to pest insects. | SyNTHESIS CLONING AND EXPRESSION IN ESCHERICHIA CQLI OF A GENE CODING FOR MCoTI-II Pham Thi Tran Chau Dao Thi Thuy Hoang Thu Ha Le Nguyen Dung Laurent Chiche 1. Introduction Proteinases are involved in different crucial living processes such as digestion germination growth development metamorphism fertilisation blood clotting infection diseases cancer etc. Therefore their protein inhibitors PPIs - an effective regulation factors have been used in medicine for treatment of diseases and in agriculture to improve plant resistance to pest insects. The dormant seeds of Cucurbitaceae plant family contain small well characterized serine proteinase inhibitors 16 23 30 . Their molecule consist of about 30 amino acids three disulfide bridges forming a compact structure resistant to the action of various biological and physico - chemical agents. Momordica cochinchinensis MCo belongs to Cucurbitaceae family. Their seeds are found to be the PPI - richest source among the tested Cucurbitaceae plants growing in Vietnam 17 21 . We have prepared a crude PPI preparation from MCo seeds named Momosertatin Mo which showed insecticidal and antibacterial activities 12 17 18 19 22 . A partly purified peptides from Mo designated as MCoPIs reduced inflammatory processes in both acute and chronic inflammatory models 14 . Moreover none toxic effect of MCoPIs was found on tested animals 14 . Three trypsin inhibitors TIs named MCoTI-I -II -III from MCo seeds were isolated 15 purified characterized and sequenced 5 . All of them are small polypeptides with molecular weight of about 3 kD and very stable. MCoTI-II is the most abudant inhibitor consisting of 34 amino acid residues with 3 disulfide bridges resistant to cleavage by thermolysin for more than 48 hours at 500C. Its conformational structure was also determined 1 10 . Although chemical synthesis of some squash TIs was found possible 11 27 but the overal procedure costly. Recombinant DNA technology was employed by several .

• Báo cáo khoa học
• Cucurbitaceae
• Momordica cochinchinensis
• blood clotting
• Scientific reports
• research projects
• scientific research
• Organic fertilizers
• Quality and yield
• Fertilizers in Cucurbitaceae family
• Cucurbitaceae family
• Use of different types
• Momordica charantia
• Bitter melon
• Medicinal plant
• Free radicals
• Plant of cucurbitaceae family
• Hoạt tính ức chế enzym α glucosidase
• Khóa luận tốt nghiệp Hóa học
• Cao hexan lá bình bát dây coccinia grandis
• Bệnh tiểu đường
• Enzym αlpha–glucosidase
• Đại cương về thực vật
• Tạp chí y học
• Nghiên cứu y học
• Cây khổ qua rừng
• Vị thuốc trị bệnh đường huyết
• Cấu trúc hóa học khổ qua rừng
• Trichosanthes cucumerina
• Trichomes and stomata
• Leaf age
• Snake tomato
• Globular headed trichomes
• Breeding system
• Cucurbita moschata
• Floral biology
• Non pseudogamous agamospermy
• Pollination ecology
• Duch
• ex Poir
• varieties
• Bitter apple
• Citrullus colocynthis (L ) Schrad (Cucurbitaceae)
• Pharmacological activities
• Brief review on bitter apple
• Khóa luận tốt nghiệp đại học
• Hóa hữu cơ
• Luận văn chuyên ngành Hóa hữu cơ
• Hoạt tính ức chế
• Enzym α glucosidase
• Lá Bình bát dây
• Coccinia grandis (L ) J Voigt
• Luận văn Thạc sĩ
• Luận văn Thạc sĩ Hoá hữu cơ
• Thành phần hoá học cây Giảo cổ lam
• Y học cổ truyền
• Bài thuốc chữa bệnh đái tháo đường
• Khóa luận tốt nghiệp
• Hóa hữu cơ
• Bình bát dây
• Hoạt tính chống oxi hóa
• Nghiên cứu dược liệu
• Phát triển dược liệu
• Khóa luận chuyên ngành Hóa hữu cơ
• Journal of Botany
• Mexican clade
• Mitochondrial DNA (CoxIII)
• Bayesian inference
• Phylogenetic perspective
• Fragment amplification
• BMC Genomics
• Gynostemma pentaphyllum
• Aerial stem to rhizome transition
• Integrated analysis
• Family Cucurbitaceae