TAILIEUCHUNG - ANALYSIS OF FOOD PRODUCTS

Food analysis is the discipline dealing with the development, application and study of analytical procedures for characterizing the properties of foods and their constituents. These analytical procedures are used to provide information about a wide variety of different characteristics of foods, including their composition, structure, physicochemical properties and sensory attributes. This information is critical to our rational understanding of the factors that determine the properties of foods, as well as to our ability to economically produce foods that are consistently safe, nutritious and desirable and for consumers to make informed choices about their diet | Analytical methods based on enzymes rely on their ability to catalyze specific reactions. These methods are rapid, highly specific and sensitive to low concentrations and are therefore ideal for determination of carbohydrates in foods. In addition, little sample preparation is usually required. Liquid foods can be tested directly, whereas solid foods have to be dissolved in water first. There are many enzyme assay kits which can be purchased commercially to carry out analysis for specific carbohydrates. Manufacturers of these kits provide detailed instructions on how to carry out the analysis. The two methods most commonly used to determine carbohydrate concentration are: (i) allowing the reaction to go to completion and measuring the concentration of the product, which is proportional to the concentration of the initial substrate; (ii). measuring the initial rate of the enzyme catalyzed reaction because the rate is proportional to the substrate concentration. Some examples of the use of enzyme methods to determine sugar concentrations in foods are given below:

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