TAILIEUCHUNG - A primary approach for separation and characterization of α-amylase from white pitaya (hylocereus undatus) peels by polymer salt two phase system

The effects of polyethylene glycol (PEG), PEG concentration, molecular weight, sodium citrate, and sodium chloride (NaCl) on yield and purification factor were studied. The highest purification factor () and yield (83%) were obtained in system PEG 6000 (14% w/w)-sodium citrate (18% w/w) with 6% (w/w) NaCl. | A PRIMARY APPROACH FOR SEPARATION AND CHARACTERIZATION OF α-AMYLASE FROM WHITE PITAYA HYLOCEREUS UNDATUS PEELS BY POLYMER SALT TWO PHASE SYSTEM Zahra Shad1 Anis Shobirin Meor Hussin1 Behrouz Akbari-adergani 2 Address es 1 Department of Food Technology Faculty of Food Science and Technology University Putra Malaysia 43400 UPM Serdang Selangor Malaysia. 2 Food and Drug Laboratory Research Center Food and Drug Administration Ministry of Health and Medical Education Tehran Iran. Corresponding author https ARTICLE INFO ABSTRACT Received 17. 7. 2020 α-Amylase was isolated from white pitaya peel with two phase system in aqueous media and then characterized. The effects of Revised 3. 9. 2021 polyethylene glycol PEG PEG concentration molecular weight sodium citrate and sodium chloride NaCl on yield and purification Accepted 6. 9. 2021 factor were studied. The highest purification factor and yield 83 were obtained in system PEG 6000 14 w w -sodium citrate Published 18 w w with 6 w w NaCl. The purified α-amylase molecular weight exhibited a band above 40 KDa by SDS-PAGE. α-Amylase showed optimum activity at pH of and temperature of 55 C. The activity of this enzyme was stable in the pH scope of 6-8 and temperature scope of 10-55 C. Various metal ions were evaluated for amylase activation inhibition effect. Na and Ca2 were exhibited Regular article to have appropriate activating effect where as Mn2 Zn2 Fe3 Ni Ba and Cu2 had inhibition effect. In addition this enzyme showed remarkable stability in the existence of TritonX-100 SDS sodium perborate Tween 20 and Tween-80. In conclusion this enzyme has high stability and activity at suitable temperature and pH and in the existence of different surfactants and metal ions. This approach has a potential in progress of various industrial applications by introducing a method for applying new low-cost and biocompatible amylases source. Keywords Aqueous two-phase system .

• Sinh học
• Biotechnology and food sciences
• Characterization of α amylase from white pitaya
• Polymer salt two phase system
• Aqueous two phase system
• Biocompatible amylases source
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