TAILIEUCHUNG - A simple automated microplate method for determining reducing sugars in food extracts and synthetic serum using cupric-neocuproine as reductant

In the present work, a simple automated microplate method based on cupric ion reduction is described for determining total reducing sugars in food extracts and synthetic serum. The reaction of Cu(II)-Nc (cupric-neocuproine) with reducing sugars was performed in alkaline medium in microplates, and the absorbance of the formed highly colored Cu(I)-Nc chelate in a plate reader at 450 nm was recorded. The proposed method was applied to reducing sugars (glucose, fructose, galactose, maltose, and lactose) and their linear calibration curves were constructed. The detection and quantification limits (LOD and LOQ) for glucose were and µM, the present work, a simple automated microplate method based on cupric ion reduction is described for determining total reducing sugars in food extracts and synthetic serum. The reaction of Cu(II)-Nc (cupric-neocuproine) with reducing sugars was performed in alkaline medium in microplates, and the absorbance of the formed highly colored Cu(I)-Nc chelate in a plate reader at 450 nm was recorded. The proposed method was applied to reducing sugars (glucose, fructose, galactose, maltose, and lactose) and their linear calibration curves were constructed. The detection and quantification limits (LOD and LOQ) for glucose were and µM, respectively. |

• Hoá học
• Journal of Chemistry
• Reducing sugars
• Glucose determination
• Microplate reader
• Fruit juice
• Cu(II) Nc spectrophotometric method
• Journal of technology and science education
• Innovative chemistry learning model
• Improving the critical thinking skill
• Self efficacy of pre service chemistry teachers
• Pre service chemistry teachers
• SCT learning model syntax covers
• The Chemistry and Antimicrobial Activity of Psychotria reevesii Wall
• The Chemistry and Antimicrobial Activity
• Psychotria reevesii Wall
• The polar fractions against Staphylococcus aureus
• The relationship between teacher related factors
• Students attitudes towards secondary school chemistry subject
• Bureti district Kenya
• Secondary school chemistry subject
• Click chemistry
• Poly(ethylene glycol)
• Poly(epsilon caprolactone)
• Azide functionalized molecules
• Azide montmorillonite clay
• Block copolymer
• Ketene chemistry
• Telechelic polymers
• Controlled radical polymerization (CRP)
• C60 fullerene
• Metallosupramolecular chemistry
• Host–guest complexes
• Supramolecular chemistry
• Donor–acceptor (D–A)
• Ring opening polymerization
• Thiol benzoxazine reaction
• Catechol based benzoxazine copolymer
• Antibacterial coatings
• Benzoxazine chemistry
• Green chemistry
• Heavy metals
• Cetylpyridinium chloride
• Analytical chemistry
• Journal of Science and Technology
• Green organic synthesis of N methylpyrrolidine
• Gas Chromatography Mass spectroscopy
• The green chemistry process
• Via the green chemistry process
• Journal of medical research
• Production of liophilized quality control samples
• Liophilized quality control samples
• Several clinical chemistry test
• Statistical quality control
• Perspectives on blended learning
• The on line platform
• LabLessons for Chemistry
• Hydrogen and Titration
• College mentors alongside
• Contextualizing technology in the classroom via remote access
• Using space exploration themes
• Scanning electron microscopy
• Tools to promote engagement
• Multidisciplinary geology chemistry experiments
• The service of learning chemistry in Industrial Engineering
• Degrees in Industrial Engineering
• Chemical Engineering and Materials Engineering
• The feed back
• Assessment of achievement in problem solving skills
• General Chemistry course
• Problem based learning
• Measure problem solving skills
• Use of chemistry software to teach
• Assess model based reaction and equation knowledge
• Assess model based reaction
• Two advanced placement
• Public suburban high school
• Collaborative activities for solving multi step problems
• Solving multi step problems in general chemistry
• Solving multi step problems
• The relatve partcipaton of instructor students
• Antioxidant activity
• Bioinorganic chemistry
• Cytotoxic activity
• DNA binding and cleavage activity
• Topoisomerase inhibitor
• Transition metal compounds
• Analytical methods
• Electroanalytical chemistry
• Ionic liquids
• Penicillin allergic patients
• Capillary electrophoresis
• Carbamate SCC interactions
• Fluorescence quenching
• Quantum chemistry calculation
• Inhibition chemiluminescence
• Piperazine chemistry
• Structural features
• Anticancer activity
• Anticancer drugs
• D cyclin dependent kinase
• 3 Amino 9 ethylcarbazole
• Multicomponent reaction
• Arylation reactions
• Cyclization reactions
• Pyrrolo[2 3 c]carbazole
• Prido[3 2 b]carbazole
• Ionic liquid
• Organic phase change materials
• Thermal energy storage
• Solvent free medium
• Silicon chemistry
• Reaction mechanism
• Density functional theory (DFT)
• Homogeneous transitionmetal catalysis
• Silicon analogues
• Medicinal chemistry
• Antiproliferative agents
• Protect endangered tissue
• Anti inflammatory cytokines
• Persistent inflammatory reaction
• Formic acid decomposition
• Renewable feedstock
• Energy feedstocks
• High surface area materials