TAILIEUCHUNG - Application of PCR technique in diagnosis of four respiratory pa thogeni c bacteria in pigs at the slaughterhouse

The polymerase chain reaction (PCR) for Actinobacillus pleuropneumonia (App), Haemophilus parasuis (Hps), Pasteurella multocida (Pm) and Bordetella bronchiseptica (Bb) were performed in pure colonies isolated from 114 lung specimens with lesions collected from the Vissan slaughterhouse in Ho Chi Minh City from July 2018 to May 2019 | Nong Lam University Ho Chi Minh City 35 Application of PCR technique in diagnosis of four respiratory pathogenic bacteria in pigs at the slaughterhouse Han M. Ly Trinh T. K. Nguyen Thiep T. X. Dang amp An T. T. Vo Department of Animal Husbandry and Veterinary Medicine Nong Lam Univsersity Ho Chi Minh Vietnam ARTICLE INFO ABSTRACT Research Paper The polymerase chain reaction PCR for Actinobacillus pleuropneumonia App Haemophilus parasuis Hps Received February 20 2019 Pasteurella multocida Pm and Bordetella bronchiseptica Revised April 26 2019 Bb were performed in pure colonies isolated from 114 Accepted May 27 2019 lung specimens with lesions collected from the Vissan slaughterhouse in Ho Chi Minh City from July 2018 to May 2019. The aim of the experiment was to identify the four respiratory pathogenic bacteria in pigs at Keywords slaughterhouse by using PCR technique. The criteria for evaluating the results included the proportion of positive samples with multiplex PCR and percentage of samples Multiplex polymerase chain reaction PCR co-infected with 2 3 and 4 bacteria. Among a total of Pig lungs 114 injured lung samples 21 of the samples was positive Respiratory bacteria to at least one of the four bacteria 3 samples Slaughterhouse were positive for App 2 samples were positive for Hps 7 samples were for Pm and 12 lungs were positive for Bb. One sample was found co-infected with Pm and Hps. Corresponding author Ly Mai Han Email 14112089@ Cited as Ly H. M. Nguyen T. T. K. Dang T. T. X. Vo A. T. T. 2019 . Application of PCR technique in diagnosis of four respiratory pathogenic bacteria in pigs at the slaughterhouse. The Journal of Agriculture and Development 18 3 35-40. 1. Introduction ing secondary infections of Haemophillus para- suis Hps and Pasteurella multocida Pm that Respiratory disease in pigs is one of the lead-normally reside in the upper respiratory tract ing concerns in the livestock industry. The major of the .

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