TAILIEUCHUNG - Generation of RNAi plasmid corresponding to the m1l1 gene (ID 140500) belonging to myosin class i in the fungus Mucor circinelloides

To investigate biological roles of Myosin class I in the morphology of M. circinelloides and identify good candidates for further analyses of Mucor pathogenesis, we are going to inhibit their expression using RNAi technique. We named the two myosin class I like genes as M1L1 (ID 140500) and M1L2 (ID 116620). In the present study, we report the results of generation of RNAi plasmid to inhibit the expression of M1L1 gene. | HNUE JOURNAL OF SCIENCE Natural Sciences 2020 Volume 65 Issue 4A pp. 27-32 This paper is available online at http GENERATION OF RNAi PLASMID CORRESPONDING TO THE m1l1 GENE ID 140500 BELONGING TO MYOSIN CLASS I IN THE FUNGUS Mucor circinelloides Le Ngoc Mai1 Le Phuong Thao1 Pham Thi Thu Trang 2 and Trieu Anh Trung1 1 Faculty of Biology Hanoi National University of Education 2 Nguyen Trai Gifted High School Hai Duong province Abstract. Mucormycosis has emerged as a second most common filamentous fungal infection in human caused by species belonging to order Mucorales especially in immunocompromised patients with a high mortality ratio. Our understanding of its virulence determinants is limited leading to the lacking of efficient therapies. Dimorphism is one of the phenotypic characteristics that can be used as a marker for virulence screening. Mucor circinelloides a dimorphic fungus has become an attractive model for studies on various studies including mucormycosis. Our previous study identified Mcmyo5 protein belonging to Myosin class V which plays an important role in Mucor pathogenesis. We have found three protein families with high similarities of amino acid sequences compared to Mcmyo5 including Myosin class I II and V in the Mucor genome. To investigate the possible roles of those genes in Mucor pathogenesis using RNAi techniques we generated the RNAi plasmid which is corresponding to endogenous gene m1l1 ID 140500 . In the present study we show the results of isolation and cloning a fragment of the target gene m1l1 into the vector pMAT1812 to obtain RNAi plasmid. This recombinant RNAi plasmid was checked by using the digestion of restriction enzyme and DNA sequencing techniques. Keywords mucormycosis myosin RNAi Mucor circinelloides. 1. Introduction Mucormycosis zygomycosis is the rare but lethal fungal infection especially in immunocompromised patients such as those with diabetes organ-transplanted AIDS hematologic malignancies or trauma .

• Sinh học
• Mucor circinelloides
• Generation of RNAi plasmid
• Myosin class
• Fungus Mucor circinelloides
• Genomic DNA
• DNA extraction
• Nấm Mucor circinelloides
• Tạo plasmid RNAi
• Gen myosin II
• Kỹ thuật RNAi
• Chủng nấm M circinelloides R7B
• Natural sciences
• Curcuma longa
• Immunocompromised patients
• Fungal infections
• Simple genomic DNA extraction
• Efficient and universal method
• The extraction of genomic DNA
• Genomic DNA from bacteria
• Microalgae suitable
• PCR based applications
• Pigeon pea
• Genomic DNA extraction
• Genomic DNA extraction from pigeon pea
• Rapid procedure
• Showed highly pure DNA
• Vietnam Journal of Science and Technology
• Optimizing dna extraction condition
• Optimizing dna extraction condition from wood
• Response surface methods
• Response Surface Methodology
• Oryza sativa
• Solanum lycopersicum
• DNA purity
• Green gram
• Liquid nitrogen
• Mungbean without liquid nitrogen
• High quality DNA extraction
• Ancient DNA
• Mainland serow
• Sequencing method
• Modern human DNA
• Rapid and efficient procedure
• Trichoderma spp
• DNA with high yield
• PCR purposes
• DNA extraction method
• Dioscorea esculenta
• Quality of DNA
• commercial kit
• Lesser yam (Dioscorea esculenta)
• Clinical Specimens
• Polymerase chain reaction
• Nucleic acid extraction
• Anti microbial resistance
• Deoxyribonucleic acid
• Cetyl trimethyl ammonium bromide
• Comparison of phenol chloroform
• Carbohydrate polymers
• Magnetic hybrid nanocomposite
• Human blood
• UV–vis absorption
• DNA BIOLOGY
• COMMERCIAL KITS
• STUTTER PRODUCTS
• DNA ANALYSIS
• MICROBIAL FORENSICS
• Commiphora wightii
• Molecular markers
• PCR amplification
• Polyvinyl pyrrolidone
• Cyprinid fishes
• Formalin preserved tissue
• Mitochondrial DNA
• Quality analysis
• Distribution and phylogenetic
• Relationships of snappers
• Phylogenetic relationships of snappers
• DNA sequence variation of the mitochondrial
• DNA isolation
• Phylogenetic analysis
• Molecular characterization of the pathogen
• Good quality fungal DNA directly
• Infected banana
• Rapid and efficient
• Extraction protocol
• Isolation of fungal DNA
• Fungal DNA
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo y học
• kiến thức y học
• nghiên cứu y học
• BMC Bioinformatics
• K mer
• Next generation sequencing
• De novo assembly
• Web server
• Redundancy control
• Quality control
• Protein coding DNA sequences
• Sequence databases increases
• BMC Genomics
• DNA sequencing
• Target sequencing panel
• Formalin fixed tissue
• Breast tissue
• Mutational signature
• Molecular barcode
• African swine fever virus
• Direct PCR
• Mutant DNA polymerase
• PCR inhibitors whole blood
• Epiretinal Prostheses
• Cell Lysis
• Measuring Methods
• MEMS Devices
• Coplanar Waveguide
• Sex determination
• Bronze Age
• Oylum Höyük
• Genetic methods
• Penaeid shrimp
• Early mortality syndrome
• Acute hepatopancreatic necrosis disease
• Bacterial diversity
• Bacterial DNA extraction
• Bacteria specific library construction