TAILIEUCHUNG - Development of a loop-mediated isothermal amplification assay for detection of tomato leaf curl new delhi virus in ridge gourd [Luffa acutangula (L) Roxb.]

Ridge gourd yellow mosaic disease (RgYMD) caused by the strain of Tomato leaf curl New Delhi virus (ToLCNDV) is an emerging disease in India. Early detection of ToLCNDV in ridge gourd has great significance in the management of the disease. An innovative loop-mediated isothermal amplification (LAMP) assay was standardized for rapid detection of ToLCNDV. Assay was carried out using the set of six primers (F3, B3, FIP, BIP, LF and BF) specific to the coat protein (CP) gene of the virus. Reaction time, temperature, primers concentration and DNA dilutions were optimized for detection of ToLCNDV. The reaction was optimized in a single tube at 63 °C for 45 min and terminated by keeping it at 80 °C for 10 min. The sensitivity of LAMP based detection assay was more than 100 times as that of conventional PCR. The LAMP primers specifically targeted to the CP gene of ToLCNDV resulted in typical water fall like bands by the agarose gel electrophoresis. The amplified LAMP products were also identified by visualization with dyes. | Development of a loop-mediated isothermal amplification assay for detection of tomato leaf curl new delhi virus in ridge gourd [Luffa acutangula (L) Roxb.]

• Nông nghiệp
• Coat protein gene
• LAMP assay
• Ridge gourd
• Yellow mosaic disease
• Ridge gourd [Luffa acutangula (L) Roxb
• Loop mediated isothermal amplification assay
• Coat protein
• Phylogenetic analysis
• Genomic RNA
• Zucchini yellow mosaic virus
• Sap transmission
• Gemini virus
• AV1 coat protein gene
• Chilli leaf curl virus
• Recombinant coat protein
• TA cloning
• His tag purification
• Cloning and expression
• Tomato leaf curl virus
• Banana bract mosaic virus
• Mysore poovan
• Molecular cloning and characterization
• Yellow mosaic virus
• Sequence comparision
• Horse gram yellow mosaic Virus
• Southern blot
• Expression vector
• Banana bunchy top viral coat protein
• Gene expression studies
• Molecular level
• Cucumber mosaic virus
• Infectious chlorosis
• Molecular characterization
• Complete coat protein gene sequence
• CMV infecting banana
• Blackgram yellow mosaic virus
• Nucleotide sequence identity
• Amino acid identity
• scientific reports
• thesis report
• medical report
• molecular Biology
• tumor cells
• Antimicrobial