TAILIEUCHUNG - Molecular cloning and characterization of coat protein gene of banana bract mosaic virus affecting banana cv. Mysore poovan (Aab)

Banana bract mosaic disease (BBrMD) is one of the most important viral diseases of banana which leads to a yield reduction. We have identified banana bract mosaic virus (BBrMV) in banana plants growing in the regions of southern India based on symptomatology, sequence homology, and Serodiagnostic assays. The viral sequence encoding the coat protein was specifically amplified by RT-PCR (Reverse Transcriptase – Polymerase chain reaction) using specific primers bordering the Coat Protein (CP) gene. The unique amplified product thus obtained was cloned into the pGEM-T vector and the authenticity of the cloned gene verified by colony PCR. The nucleotide sequences and the deduced amino acid sequences were compared with the other BBrMV isolates and found to be identical at both the nucleotide and amino acid sequence level of other isolates with 99 to 96 per cent and 95 to 83 per cent respectively. The phylogenetic analysis by the alignment of CP gene sequences of selected 22 isolates also revealed that the present isolate was more similar to KER2 (Kasaragod) isolate. The recombinant clones developed in the present study could be applied in serodiagnosis and genetic engineering. This could be also used as disease diagnostic probes for more sensitive molecular diagnostic techniques like Nucleic acid spot hybridization. | Molecular cloning and characterization of coat protein gene of banana bract mosaic virus affecting banana cv. Mysore poovan (Aab)

• Sinh học
• Banana bract mosaic virus
• Coat protein gene
• Mysore poovan
• Banana bract mosaic virus
• Molecular cloning and characterization
• BMC Plant Biology
• Indigo alkaloids
• Baphicacanthus cusia
• 5 enolpyruvylshikimate 3 phosphate synthase
• Molecular cloning
• Enzyme assay
• Plant metabolic engineering
• Bacillus thuringiensis
• Characterization analysis
• Gene cloning
• Escherichia coli
• Polymerase chain reaction
• Melon Cucumis melo L
• Ran binding protein
• Gene expression
• Heat stress
• Cholinergic system
• Molecular structure of acetylcholinesterase
• Recombinant protein
• Enzyme characterization
• Biochemical properties of the enzyme
• Report medicine
• traditional medicine
• scientific reports
• medical knowledge
• cytoplasm
• analysis of cytoplasmic
• molecular Biology
• tumor cells
• Antimicrobial
• biochemistry
• Bacillus
• biochemical studies
• environmental medicine
• Crystal structure
• Báo cáo sinh học hay
• cách trình bày báo cáo
• báo cáo sinh học
• công trình nghiên cứu sinh học
• tài liệu về sinh học
• medical report
• scientific research
• medical analysis
• oxidation
• chemical reaction
• medicine
• cell proliferation
• medical research
• hemoglobin
• Diannan small ear pig
• Arrestin domain containing 1
• Arrestin domain containing 5
• Arrestin family
• Tissue expression profile
• Japanese morning glory
• Ipomoea nil
• Heavy metal
• Micronutrient homeostasis
• Salt tolerance
• Spartina anglica
• Transgenic rice
• Rapid amplification of cDNA ends
• Transgenic plants
• O methyltransferase
• S adenosyl L methionine dependent
• Citrus depressa
• Anti pathogenic properties
• Plant Biology
• Gossypium hirsutum
• Gland formation
• Transcription factor
• Biofuel products
• Darkly pigmented glands
• Non ruminant animals
• BMC Genomics
• Vitis vinifera
• Serotonin N acetyltransferase
• Ubiquitous molecule
• Melatonin biosynthetic pathway